393206

Anti-HNE Fluorophore Rabbit pAb

liquid, Calbiochem^®

• Synonyme(s) : Anti-N ^α-Acetyllysine-4-hydroxy-2-nonenal Fluorophore, Anti-N^α-Acetyllysine-4-hydroxy-2-nonenal Fluorophore
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: diluted serum
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: liquid
• Réactivité de l'espèce (prédite par homologie): all
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; avoid repeated freeze/thaw cycles
• Isotype: IgG
• Conditions d'expédition: wet ice
• Température de stockage: −70°C
• Modification post-traductionnelle de la cible: unmodified

Description

Description générale

Anti-HNE Fluorophore, rabbit polyclonal, recognizes the HNE-derived chromphohore of the 2:1 lysine-HNE-fluorophore. It is validated for use in ELISA, Western blotting, and immunohistochemistry.

Rabbit polyclonal antibody supplied as diluted serum. Recognizes the HNE-derived chromophore of the 2:1 lysine-HNE fluorophore.

Recognizes the HNE-derived chromphohore of the 2:1 lysine-HNE-fluorophore.

Immunogène

a 2:1 N^α-acetyllysine-HNE fluorophore, conjugated to KLH

Application

ELISA (1:500-1:1000)

Immunoblotting (1:1000-1:5000)

Immunohistochemistry (1:100-1:500)

Avertissement

Toxicity: Standard Handling (A)

Forme physique

Serum diluted 1:1 with glycerol.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Autres remarques

Due to the small epitope size, overnight incubation with the antibody greatly increases sensitivity by immunoblotting. Determined by competitive ELISA and immunoblotting to recognize the chromophore structure of fluorophore derived from the reaction of 4-HNE with primary amines. Does not recognize 1:1 cysteine-, 1:1 histidine-, or 1:1 lysine-HNE Michael Adducts. Variables associated with assay conditions will dictate the proper working dilution.

Tsai, L., et al. 1998. Proc. Natl. Acad. Sci. USA95, 7975.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1