401105

Importazole

A cell-permeable diaminoquinazoline compound that selectively blocks importin-β-mediated nuclear import of NLS bearing cargos in a reversible manner.

• Synonyme(s) : Importazole, Importin-β Inhibitor, N-(1-Phenylethyl)-2-pyrrolidin-1-ylquinazolin-4-amine, IPZ, N-(1-Phenylethyl)-2-pyrrolidin-1-ylquinazolin-4-amine, IPZ, Importin-β Inhibitor
• Formule empirique (notation de Hill) : C₂₀H₂₂N₄
• CAS Number: 662163-81-7
• Poids moléculaire : 318.42
• Numéro MDL: MFCD04077630
• Code UNSPSC : 12352200
• ID de substance PubChem : 2949965
• Nomenclature NACRES : NA.51

Propriétés

• Niveau de qualité: 100
• Essai: ≥98% (HPLC)
• Forme: solid
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; protect from light
• Couleur: white
• Solubilité: DMSO: 25 mg/mL, clear, colorless
• Conditions d'expédition: ambient
• Température de stockage: −20°C
• Chaîne SMILES: CC(C1=CC=CC=C1)NC2=NC(=NC3=CC=CC=C32)N4CCCC4
• Clé InChI: HKGJEZIGDHFJFL-UHFFFAOYSA-N

Description

Description générale

A cell-permeable diaminoquinazoline compound that selectively blocks importin-β-mediated nuclear import of NLS- (nuclear localization signal) bearing cargos (IC₅₀ = 15 µM against Ca⁺²-induced NFAT nuclear import in HEK293 cells) in a reversible manner without affecting transportin-dependent nuclear import of M9 signal-containing cargos or CRM1-mediated nuclear export. IPZ affects, but not blocks, importin-β-RanGTP interaction, likely by inducing a conformational change upon direct importin-β binding. IPZ treatment impairs cellular mitosis and is cytotoxic to HeLa cells (50% and >90% killing in 24 h, respectively, with 22.5 and 60 µM IPZ).

Conditionnement

Packaged under inert gas

Avertissement

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Autres remarques

Soderholm, J.F., et al. 2011. ACS Chem. Biol.6, 700.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable