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407294

Sigma-Aldrich

αA-Interferon, Human, Recombinant, E. coli

Synonyme(s) :

IFNA2A, IFNA2B, IFNA2C, rhIFN-αA

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About This Item

Code UNSPSC :
12352202
Nomenclature NACRES :
NA.77
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Description

Merck USA index - 14, 4990

Niveau de qualité

Essai

≥95% (SDS-PAGE)

Forme

liquid

Activité spécifique

≥2.8 x 108 units/mg protein
≥5 x 106 units/mL

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

OK to freeze
avoid repeated freeze/thaw cycles

Technique(s)

cell based assay: suitable

Numéro d'accès NCBI

Numéro d'accès UniProt

Comparer avec des articles similaires

Voir la comparaison complète

Montrer les différences

1 of 4

Cet article
407306407320616374
assay

≥95% (SDS-PAGE)

assay

-

assay

≥95% (RP-HPLC, FPLC, and SDS-PAGE)

assay

≥95% (SDS-PAGE)

technique(s)

cell based assay: suitable

technique(s)

-

technique(s)

-

technique(s)

-

form

liquid

form

liquid

form

lyophilized

form

liquid

UniProt accession no.

P01563

UniProt accession no.

-

UniProt accession no.

-

UniProt accession no.

-

specific activity

≥2.8 x 108 units/mg protein, ≥5 x 106 units/mL

specific activity

-

specific activity

-

specific activity

-

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

OK to freeze, desiccated

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

Description générale

Research area: Immunoand cks

Interferons are cytokines secreted by mammalian cells. They are broadly divided into type I– interferon alpha (IFN-α) and interferon beta (IFN-β), type II – interferon-gamma (IFN-γ), and type III – interferon lambda (IFN-λ) based on their structure, function, and receptor specificity. Recombinant αA interferon is a hydrophobic protein belonging to the IFN-α family of proteins.[1]

Application

Interferon alpha has been used to evaluate its effect on VSV (vesicular stomatitis virus) infection in pancreatic ductal adenocarcinoma (PDA) cell lines and study the relationship between type I IFNsignaling, resistance to VSV infection, and the JAK/STAT (Janus kinase/signal transducers and activators of transcription) signaling pathways in PDA cell lines.[2] It has also been used to study the inhibitory effect of Morbillivirus V Proteins on Interferon Signaling Pathways.[3]

Actions biochimiques/physiologiques

Recombinant, human αA-interferon expressed in E. coli have antiviral, antiproliferative, antineoplastic, and immunomodulating properties. Recombinant interferon αA (IFN-αA) is used as the first line of treatment for hepatitis C.[1] It also displays a great therapeutic potential with chronic viral hepatitis, polycythemia, leukemia, and various other malignancies. It enhances natural killer cell activity, suppresses bone marrow function, and induces apoptotic cell death in non-melanoma skin cancer.

Avertissement

Toxicity: Standard Handling (A)

Définition de l'unité

One unit of interferon per ml of medium will cause a 50% inhibition of VSV-induced cytopathic effect on bovine MDBK cells. Units are determined with respect to the NIH international reference standard for human αA-IFN. Dilute in aqueous buffer containing at least 0.1% BSA or HSA.

Forme physique

In PBS containing 0.1% BSA.

Notes préparatoires

Dilute only in buffers containing protein such as 0.1% BSA.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Autres remarques

Fujishima, H., et al. 1998. Int. J. Cancer 76, 423.
Haria, M., and Benfield, P. 1995. Drugs 50, 873.
Familletti, P.C., et al. 1981. Methods Enzymol. 78, 387.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Wouter Huvenne et al.
Respiratory research, 11, 100-100 (2010-07-24)
Cigarette smoke (CS) is known to initiate a cascade of mediator release and accumulation of immune and inflammatory cells in the lower airways. We investigated and compared the effects of CS on upper and lower airways, in a mouse model
N F Li et al.
Cell proliferation, 40(5), 780-794 (2007-09-20)
Cell immortalization is considered to be a prerequisite status for carcinogenesis. Normal human ovarian surface epithelial (OSE) cells, which are thought to be the origin of most of human ovarian carcinomas, have a very limited lifespan in culture. Establishment of
Vijay G Ramakrishnan et al.
Journal of biomolecular techniques : JBT, 16(4), 297-305 (2006-03-09)
Ticks are obligate ectoparasites that feed on a variety of hosts including mammals, birds and reptiles. Prolonged attachment on the host and an ability to transmit a wide variety of pathogens are the special features of tick feeding. Salivary glands
M Otsuka et al.
The Journal of biological chemistry, 275(44), 34122-34130 (2000-08-05)
Hepatitis C virus (HCV) causes a persistent infection, chronic hepatitis, and hepatocellular carcinoma. Since there are several reports indicating that some viruses influence the tumor suppressor p53 function, we determined the effects of HCV proteins on p53 function and its
Axel Hoos et al.
The American journal of pathology, 160(1), 175-183 (2002-01-12)
Hürthle cell tumors are rare thyroid neoplasms for which disease biology is poorly understood and diagnosis of carcinoma can be challenging. The aim of the study was to characterize molecular expression profiles of Hürthle cell tumors and to determine the

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