537059

Propidium Iodide

Membrane impermeable DNA intercalator.

• Synonyme(s) : Propidium Iodide
• Formule empirique (notation de Hill) : C₂₇H₃₄I₂N₄
• CAS Number: 25535-16-4
• Poids moléculaire : 668.39
• Numéro MDL: MFCD00011921
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.84

Propriétés

• Niveau de qualité: 100
• Description: RTECS - SF7949600
• Forme: solid
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; protect from light
• Couleur: dark red
• Entrée: sample type intact cells
• Fluorescence: λ_ex ~536 nm; λ_em ~617 nm
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• Chaîne SMILES: [I-].I.[N+](CCCN1C2=CC(=N)C=CC2=C3C(=C1c4ccccc4)C=C(C=C3)N)(CC)(CC)C
• InChI: 1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1
• Clé InChI: XJMOSONTPMZWPB-UHFFFAOYSA-M

Description

Description générale

Extinction coefficient(493 nm): 5900 M ^-1cm^-1;(287 nm): 55,700 M ^-1cm^-1;

Membrane impermeable DNA intercalator. Has red fluorescence at 488 nm. Useful for staining apoptotic cells and nuclei by flow cytometry. Can be used to differentiate between apoptotic and necrotic cell death. Can be used together with common green fluorescent probes labeled with fluorescein isothiocyanate. Has the following spectral properties: extinction coefficient: 5,900 M^-1cm^-1 (493 nm); 55,700 M^-1cm^-1 (287 nm); 13,800 M^-1cm^-1 (252.5 nm); excitation maximum: 536 nm; emission maximum: 617 nm.

Actions biochimiques/physiologiques

Cell permeable: no

Primary Target
Membrane impermeable DNA intercalator

Product does not compete with ATP.

Reversible: no

Avertissement

Toxicity: Irritant (B)

Notes préparatoires

Solubility may be improved by first dissolving in a very small amount of DMSO, then adding material to an aqueous solution.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Autres remarques

Belloc, F., et al. 1994. Cytometry 17, 59.
Crompton, T., et al. 1992. Biochem. Biophys. Res. Commun. 183, 532.
Darzynkiewicz, Z., et al. 1992. Cytometry13, 795.
de Caestecker, M.P., et al. 1992. J. Immunol. Methods154, 11.
Pollice, A.A., et al. 1992. Cytometry13, 432.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Warning
• Mentions de danger: H341
• Conseils de prudence: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Classification des risques: Muta. 2
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable