604980

Thrombin, Bovine, High Activity

• Synonyme(s) : Thrombin, Bovine, High Activity
• CAS Number: 9002-04-4
• Numéro MDL: MFCD20921901
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.25

Propriétés

• Source biologique: bovine
• Niveau de qualité: 100
• Description: Merck USA index - 14, 9383
• Forme: lyophilized
• Activité spécifique: ≥1800 NIH units/mg protein
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze
• Solubilité: water: soluble
• Conditions d'expédition: ambient
• Température de stockage: −20°C

Description

Description générale

Thrombin is a sodium-activated type II enzyme. This serine protease enzyme consists of two anion binding exosites, ABE-I and ABE-II. Thrombin is synthesized from zymogen prothrombin (factor II) in the liver. Bovine A-thrombin comprises a light chain (A chain) and a heavy chain (B chain). These two chains are linked by non-covalent interactions and by one disulfide bond.

Application

Thrombin, bovine, high activity has been used:

• to stimulate LNCaP cell and to study the effect of activation of G-protein-coupled receptors (GPCRs)
• in fibrin gel fabrication
• to form fibrinogen gel by fibrinogen gel-based method

Actions biochimiques/physiologiques

Thrombin converts fibrinogen into fibrin and activates factors V, VIII, XI, and XIII. It promotes platelet activation and vascular contraction. Thrombin stabilizes the fibrin polymers. It plays a crucial role in the final stages of the blood coagulation cascade.

Avertissement

Toxicity: Harmful (C)

Forme physique

Lyophilized from 200 mM NaCl, 50 mM sodium citrate, 1 ml 0.1% PEG, pH 6.5.

Remarque sur l'analyse

Complete activation from homogeneous prothrombin by SDS-PAGE

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Pictogrammes: GHS08,GHS07
• Mention d'avertissement: Danger
• Mentions de danger: H315,H319,H334,H335
• Conseils de prudence: P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
• Classification des risques: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable