627610

Z-VAD-FMK

Single spot by TLC, solid, Caspase inhibitor, Calbiochem

• Synonyme(s) : Caspase Inhibitor I, Z-VAD (OMe)-FMK
• Formule empirique (notation de Hill) : C₂₂H₃₀FN₃O₇
• CAS Number: 187389-52-2
• Poids moléculaire : 467.49
• Numéro MDL: MFCD02684037
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.77

Propriétés

• Product Name: Caspase Inhibitor I, Z-VAD-FMK, CAS 187389-52-2, is a cell-permeable, irreversible, pan-caspase inhibitor.
• Niveau de qualité: 100
• Forme: solid
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; desiccated
• Couleur: white
• Solubilité: DMSO: 50 mM
• Conditions d'expédition: ambient
• Température de stockage: −20°C
• Chaîne SMILES: FCC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)OCc1ccccc1)C(C)C)C)CC(=O)OC
• InChI: 1S/C22H30FN3O7/c1-13(2)19(26-22(31)33-12-15-8-6-5-7-9-15)21(30)24-14(3)20(29)25-16(17(27)11-23)10-18(28)32-4/h5-9,13-14,16,19H,10-12H2,1-4H3,(H,24,30)(H,25,29)(H,26,31)/t14-,16-,19-/m0/s1
• Clé InChI: MIFGOLAMNLSLGH-QOKNQOGYSA-N

Description

Description générale

A cell-permeable, irreversible, pan-caspase inhibitor. Shown to enhance the freeze-thaw survival of human embryonic stem cells. Inhibits Fas-mediated apoptosis in Jurkat T cells. Also reported to inhibit Peptide: N-glycanse (PNGase) in vitro amd in vivo. When using with a purified recombinant enzyme, pretreatment with an esterase is required.

Actions biochimiques/physiologiques

Cell permeable: yes

Primary Target
Fas-mediated apoptosis in Jurkat cells and staurosporine-induced cell death in corneal epithelial cells

Product does not compete with ATP.

Reversible: no

Avertissement

Toxicity: Standard Handling (A)

Séquence

Z-Val-Ala-Asp(OMe)-CH₂F*

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for 6 months at -20°C.

Remarque sur l'analyse

Single spot by TLC

Autres remarques

Heng, B.C., et al. 2007. Biosci. Rep.27, 257.
Martin, U., et al. 2007. Apoptosis12, 525.
Misaghi, S., et al. 2004. Chem. Biol.11, 1677.
Joo, C., et al. 1999. Ophthalmic Res. 31, 287.
McColl, K.S., et al. 1998. Mol. Cell. Endocrinol. 139, 229.
Thornberry, N.A. and Lazebnik, Y. 1998. Science 281, 1312.
Tolomeo, M., et al. 1998. Cell Death Differ. 5, 735.
An, S. and Know, K.A. 1996. FEBS Lett.386, 115.
Cain, K., et al. 1996. Biochem J.314, 27.
Mirzoeva, O.K., et al. 1996. FEBS Lett.396, 266.
Pronk, G.J., et al. 1996. Science 271, 808.
Slee, E.A., et al. 1996. Biochem. J.315, 21.
Chow, S.C., et al. 1995. FEBS Lett. 364, 134.
Fearnhead, H.O., et al. 1995. FEBS Lett. 375, 283.
Zhu, H., et al. 1995. FEBS Lett.374, 303.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable