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Vascular Endothelial Growth Factor 165 human

animal free, >98% (SDS-PAGE and HPLC), recombinant, expressed in E. coli, suitable for cell culture

Synonyme(s) :

Vascular Endothelial Growth Factor 165, VEGF-165, VEGF, Vascular Endothelial Growth Factor, VEGF-A

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About This Item

Code UNSPSC :
12352202
eCl@ss :
32160405
Nomenclature NACRES :
NA.77
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Product Name

VEGF165 Protein, Human Recombinant Animal Free, The Vascular endothelial growth factor (VEGF or VEGFA) is a potent mediator of both angiogenesis & vasculogenesis in the fetus & adult. Manufactured using all non-animal reagents.

Niveau de qualité

Essai

>98% (SDS-PAGE and HPLC)

Impuretés

<0.1 μg/mg protein endotoxin (<1EU/μg)

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Description générale

Product Source: E. coli
Vascular endothelial growth factor (VEGF or VEGFA) is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. Humans express alternately spliced isoforms of 121, 145, 165, 183, 189, and 206 amino acids (aa) in length. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189. VEGF is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, VEGF plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates VEGF in the induction of tumor metastasis and intra-ocular neovascular syndromes. VEGF signals through the three receptors; fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product. Recombinant human VEGF is a 38.2 kDa disulfide-linked homodimeric protein consisting of two 165 amino acid polypeptide chains.

Spécificité

Cross Reactivty
Mouse, Rat, Pig, Hamster, Trout, Green Algae, Sheep, Cow, Rabbit, Guinea Pig

Application

Research Category
Stem Cell Research
Research Sub Category
Growth Factors & Receptors

Qualité

Bioactivity assay: Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1.0-8.0 ng/ml.

Forme physique

Product is filtered through a 0.2 micron filter before lyophilization.
Recombinant animal free human VEGF165 is manufactured using all non-animal reagents

Stockage et stabilité

Store at -20°C for up to 6 months from date of receipt.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Dante Pascual Goy et al.
The open biomedical engineering journal, 6, 85-91 (2012-08-01)
At present, typical approaches employed to repair fractures and other bone lesions tend to use matrix grafts to promote tissue regeneration. These grafts act as templates, which promote cellular adhesion, growth and proliferation, osteoconduction, and even osteoinduction, which commonly results
Danielle Wu et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(29), 12096-12101 (2013-07-03)
Osteocytes are bone cells that form cellular networks that sense mechanical loads distributed throughout the bone tissue. Interstitial fluid flow in the lacunar canalicular system produces focal strains at localized attachment sites around the osteocyte cell process. These regions of
Chao-Lin Chen et al.
Scientific reports, 9(1), 10616-10616 (2019-07-25)
Autologous vascular grafts have the advantages of better biocompatibility and prognosis. However, previous studies that implanted bare polymer tubes in animals to grow autologous tubular tissues were limited by their poor yield rates and stability. To enhance the yield rate
Sophie Lanone et al.
Particle and fibre toxicology, 6, 14-14 (2009-05-02)
A critical issue with nanomaterials is the clear understanding of their potential toxicity. We evaluated the toxic effect of 24 nanoparticles of similar equivalent spherical diameter and various elemental compositions on 2 human pulmonary cell lines: A549 and THP-1. A
Balmiki Ray et al.
Brain research, 1297, 160-168 (2009-08-08)
Cholinergic neurons are a major constituent of the mammalian central nervous system. Acetylcholine, the neurotransmitter used by cholinergic neurons, is synthesized from choline and acetyl CoA by the enzymatic action of choline acetyltransferase (ChAT). The transport of choline into the

Protocoles

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