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ChemiSCREEN Membrane Preparation Recombinant Human CB2 Cannabinoid Receptor

Human CB2 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.

Synonyme(s) :

Cannabinoid receptor membrane prep, Human CB2 membrane prep

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5 G
13 520,00 INR

13 520,00 INR


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5 G
13 520,00 INR

About This Item

Code UNSPSC :
41106514
eCl@ss :
32161000
Nomenclature NACRES :
NA.41

13 520,00 INR


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Source biologique

human

Niveau de qualité

Produit recombinant

expressed in CHO cells

Fabricant/nom de marque

ChemiScreen
Chemicon®

Technique(s)

ligand binding assay: suitable (GTPγS)
radioligand binding assay (RLBA): suitable

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Comparer avec des articles similaires

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1 of 4

Cet article
Y1375MHA000P2MMHA00PRY2
YPD Broth suitable for microbiology, NutriSelect® Basic

Y1375

YPD Broth

feature

ready-to-use

feature

-

feature

ready-to-use

feature

ready-to-use

packaging

pack of 50 tests

packaging

poly bottle of 1 kg, poly bottle of 250 g

packaging

pack of 50 tests

packaging

pack of 50 tests

suitability

molds, yeasts

suitability

nonselective for Candida spp., nonselective for Pichia spp., nonselective for Saccharomyces spp., nonselective for Zygosaccharomyces spp.

suitability

molds, yeasts

suitability

yeasts

form

liquid

form

powder

form

liquid

form

liquid

application(s)

clinical
cosmetics
environmental
food and beverages
industrial qc (microbial monitoring)
microbiology

application(s)

environmental
food and beverages, microbiology

application(s)

clinical
cosmetics
environmental
food and beverages
industrial qc
water monitoring, microbiology

application(s)

bioburden testing
clinical
cosmetics
environmental
food and beverages
industrial qc (microbial monitoring)
microbiology
water monitoring

shipped in

wet ice

shipped in

-

shipped in

wet ice

shipped in

wet ice

Description générale

Cannabinoid compounds include exogenous drugs such as D9-THC, the main psychoactive component of the plant Cannabis sativa, and endogenous mediators, such as anandamide, that belong to eicosanoid family.The biological effects of cannabinoids are mediated by a family of two Gi-coupled 7-transmembrane receptors, CB1 and CB2. The CB1 receptor is found primarily in brain and mediates the psychoactive effects of cannabinoid ligands. The CB2 receptor is expressed mainly in immune cells, including mast cells and CD40-activated B cells, where it mediates proliferation and inhibition of migration (Howlett et al., 2002). Activation of CB2 inhibits the development of liver fibrosis (Julien et al., 2005). In bone, CB2 is expressed in both osteoblasts and osteoclasts, and functions to prevent bone loss (Ofek et al., 2006). In addition, activation of CB2 has an antinociceptive effect in animal models of neuropathic, inflammatory, and acute pain; this effect is mediated by release of endogenous opioids in the periphery (Ibrahim et al., 2005). Millipore′s CB2 membrane preparations are crude membrane preparations made from stable recombinant cell lines with a high-level of GPCR surface expression; thus, they are ideal HTS tools for screening for agonists and antagonists of CB2. The membrane preparations exhibit a Kd of 2.7 nM for [3H]-CP55,940. With 5 μg/well CB2 Membrane Prep and 2.5 nM [3H]-CP55,940, greater than 10-fold signal-to-background ratio is obtained.
Full-length Human CB2

Qualité

Table 1. Signal:background and specific binding values obtained in a competition binding assay with varying amounts of CB2 membrane prep.
10 µg/well 5 µg/well
Signal:Background 33.5 25.2
Specific Binding (cpm) 7336 6207

SPECIFICATIONS: 1 unit = 5 μg membrane preparation
Bmax: 50 pmol/mg
Kd: 2.7 nM

Caractéristiques

Inucbation Conditions
RECOMMENDED ASSAY CONDITIONS: Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, an FC 96-well harvest plate (Millipore cat. # MAHF C1H) is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.

Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C.

Radioligand: [3H]-CP55940 (Perkin Elmer #NET1051)

Wash Buffer: 50 mM Hepes, pH 7.4, 500 mM NaCl , 0.1% BSA, filtered and stored at 4°C.

One package contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 10-fold signal:background with 3H-labeled CP55,940 at 2.5 nM

Forme physique

Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA no preservatives.
Packaging method: Membranes proteins were adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Synthesis of 1-(n-hexyl-5-one)-2-chlorobenzimidazole.
Dubey PK, et al.
Indian J. Chem. B, 44, 1239-1242 (2005)
Chimie organometallique sous haute pression: Reaction des chlorocetones avec l'hydrure de tributyletain.
Degueil-Castaing M, et al.
Journal of Organometallic Chemistry, 287(1), 49-56 (1985)

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