IP02

Protein A Agarose Suspension

Protein A Agarose Suspension designed for immunoprecipitation applications

• Code UNSPSC : 41116133
• Nomenclature NACRES : NA.56

Propriétés

• Forme: slurry (Liquid)
• Contient: ≤0.1% sodium azide as preservative
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: do not freeze
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C

Description

Description générale

Designed for immunoprecipitation applications. This product is blocked with BSA to reduce non-specific binding and cannot be used for purification; best for mouse IgG_2a and IgG_2b and rabbit IgG.

Protein A conjugated to agarose.

Avertissement

Toxicity: Standard Handling (A)

Forme physique

In PBS.

Autres remarques

Agarose solutions are supplied ready-to-use for immunoprecipitation. Use 15 µl suspension/µg primary antibody. Protein A-Agarose is recommended for use in immunoprecipitation assays using rabbit polyclonal antibodies or unmodified mouse IgG_2a or IgG_2b monoclonal antibodies. Mouse IgG₁ or IgG₃ and all rat antibodies bind poorly to Protein A. As an alternative, use Protein G PLUS, Protein G PLUS/Protein A, or rabbit second-step antibodies to bridge these antibodies to Protein A. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl of agarose conjugate and 1 µg of normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable