NA81

Anti-5-Methylcytosine Mouse mAb (162 33 D3)

liquid, clone 162 33 D3, Calbiochem^®

• Synonyme(s) : Anti-5-mc, Anti-5-MeCyd
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: 162 33 D3, monoclonal
• Forme: liquid
• Ne contient pas: preservative
• Réactivité de l'espèce (prédite par homologie): all
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; avoid repeated freeze/thaw cycles
• Isotype: IgG1
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified

Description

Description générale

Immunoaffinity purified mouse monoclonal antibody. Recognizes 5-methylcytosine.

Recognizes 5-methylcytosine in methylated DNA or RNA in NIH3T3 cells.

Specific
• Affinity purified mouse monoclonal antibody - Detects methylated DNA from a broad range of species. Several Applications
• Flow Cytometry
• Frozen Sections
• Immunoblotting
• Immunofluorescence
• Paraffin Sections

Reliable
• Generates reproducible results.
• Clone 162 33 D3 has been used in over 50 publications.

This Anti-5-Methylcytosine Mouse mAb (162 33 D3) is validated for use in ELISA, FC, Frozen Sections, IF, Paraffin Sections, Radioimmunoassay, Southwestern Blot for the detection of 5-Methylcytosine.

Immunogène

5-methylcytosine conjugated to ovalbumin

Application

ELISA (0.1 g/ml)
Flow Cytometry (1 g/ml)
Frozen Sections (5-10 g/ml)
Immunoblotting (5 g/ml)
Immunofluorescence (1 g/ml)
Paraffin Sections (5-10 g/ml)
Radioimmunoassay (0.1 g/ml)
Southwestern Blot (5 g/ml)

Conditionnement

Please refer to vial label for lot-specific concentration.

Avertissement

Toxicity: Standard Handling (A)

Forme physique

In PBS, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Remarque sur l'analyse

Positive Control
NIH3T3 cells

Autres remarques

Clone 162 33 D3 is useful for the quantitative and qualitative detection of methylated DNA or RNA in a variety of samples and applications. It has been used in over 40 publications. Antibody should be titrated for optimal results in an individual systems.

Ehrlich, M. 2002. Oncogene21, 5400. (Review)
Widschwendter, M. and Jones, P.A. 2002. Oncogene21, 5462. (Review)
Barton, S.C., et al. 2001. Hum. Mol. Genet.10, 2983.
Piyathilake, C.J., et al. 2001. Hum. Pathol.32, 856.
Taddei, A., et al. 2001. Nat. Cell Biol.3, 114.
Piyathilake, C.J., et al. 2000. Biotech. Histochem.75, 251.
De Capoa, A., et al. 1999. FASEB J.13, 89.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable