OP20

Anti-c-Abl (Ab-3) Mouse mAb (24-21)

liquid, clone 24-21, Calbiochem^®

• Synonyme(s) : Anti-p150, Anti-Abl
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 24-21, monoclonal
• Forme: liquid
• Contient: ≤0.1% sodium azide as preservative
• Espèces réactives: mouse, human
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: do not freeze
• Isotype: IgG1
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... ABL1(25)

Description

Description générale

Anti-c-Abl (Ab-3), mouse monoclonal, clone 24-21, recognizes the ~145 kDa human and ~150 kDa mouse c-Abl. It is validated for Western blotting, immunofluorescence, and immunoprecipitation.

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with p.3U1 mouse myeloma cells (see application references). Recognizes the v-Abl, the ~145-150 kDa c-Abl, the ~210 kDa Bcr-Abl (CML), and the ~190 kDa Bcr-Abl (ALL) proteins.

Recognizes the ~145 kDa human c-Abl, the ~150 kDa mouse c-Abl, v-Abl, and the Bcr/Abl translocation products in ALL (~190 kDa) and CML (~210 kDa). Does not inhibit protein tyrosine kinase activity.

Antibody Target Gene Symbol: ABL1

Target Synonym: ABL, AI325092, bcr/abl, C-ABL, C-ABL 1B, CABL1, E430008G22Rik, JTK7, MGC117749, p145Abl, p150, v-abl

Entrez Gene Name: c-abl oncogene 1, receptor tyrosine kinase

Hu Entrez ID: 25 (Related Antibodies: PK1006PK1013OP19)

Mu Entrez ID: 11350

Rat Entrez ID: 311860

Immunogène

a recombinant protein consisting of the carboxyl region of v-abl protein fused to TrpE

Application

Immunoblotting (1-5 µg/ml)

Immunofluorescence (see application references)

Immunoprecipitation (1-2 µg/sample)

Neutralization Studies (not recommended)

Conditionnement

Please refer to vial label for lot-specific concentration.

Avertissement

Toxicity: Standard Handling (A)

Forme physique

In 0.05 M sodium phosphate buffer, 0.2% gelatin, pH 7.5.

Remarque sur l'analyse

Positive Control
K562 (human bcr/abl), HL-60 (normal abl), and ANN-1 (murine abl) cells

Autres remarques

Does not inhibit protein tyrosine kinase activity. Detects human c-Abl (145 kDa), mouse c-Abl (150 kDa), v-Abl, and the Bcr/Abl translocation products in ALL (~210 kDa) and CML (~190 kDa). HL-60 and NIH3T3 cells contain normal, non-rearranged protein. Antibody should be titrated for optimal results in individual sample types.

Wiedemann, L.M., et al. 1988. Blood71, 349.
Stam, K., et al. 1985. N. Engl. J. Med.313, 1429.
Konopka, J.B., et al. 1984. Cell37, 1035.
Prywes, R., et al. 1983. Cell34, 569.
de Klein, A., et al. 1982. Nature300, 765.
Reynolds, F.H., Jr., et al. 1980. J. Virol.36, 374.
Reynolds, F.H., Jr., et al. 1978. Proc. Natl. Acad. Sci. USA75, 3974.
Witte, O.N., et al. 1978. Proc. Natl. Acad. Sci. USA75, 2488.
Abelson, H.T. and Rabstein, L.S. 1970. Cancer Res.30, 2213.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable