10280879001

5-bromo-2′-désoxyuridine

>97%, crystalline solid, pkg of 1 g

• Synonyme(s) : 5-BrdU, 5-bromo-1-(2-désoxy-β-D-ribofuranosyl)uracile, 5-bromo-uracile-désoxyriboside, BUdR
• Formule empirique (notation de Hill) : C₉H₁₁BrN₂O₅
• CAS Number: 59-14-3
• Poids moléculaire : 307.10
• Beilstein: 30395
• Numéro MDL: MFCD00006529
• Code UNSPSC : 12352204
• ID de substance PubChem : 329749004

Propriétés

• Niveau de qualité: 100
• Essai: >97%
• Forme: crystalline solid
• Poids mol.: 307.1
• Conditionnement: pkg of 1 g
• Fabricant/nom de marque: Roche
• Pf: 191-194 °C (dec.) (lit.)
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C
• Chaîne SMILES: OC[C@H]1O[C@H](C[C@@H]1O)N2C=C(Br)C(=O)NC2=O
• InChI: 1S/C9H11BrN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)/t5-,6+,7+/m0/s1
• Clé InChI: WOVKYSAHUYNSMH-RRKCRQDMSA-N

Description

Description générale

It is conventionally used as a marker for S-phase synthesis of DNA.

Application

5-Bromo-2′-deoxyuridine has been used to label cells in the S-phase of the cell cycle and primary hepatocytes during cell proliferation studies.

Immunochemically detectable nucleotide that can be incorporated into DNA instead of thymidine. Used for in vivo studies of DNA synthesis and for the detection of mutagenic substances, according to the sister chromatid exchange (SCE) method.

Actions biochimiques/physiologiques

Il s'agit d'un analogue de la thymidine utilisé comme agent mutagène en recherche génétique. Il permet une incorporation sélective dans l'ADN cellulaire au cours de la phase S.

Qualité

Purity: 97% (from N), chromatographically homogeneous

Notes préparatoires

Working concentration: According to literature for cell culture, a final concentration of 10 μmol/l has been used.
According to literature, 5 to 50 mg/kg body weight mice have been used for the detection of cell proliferation.

Preparation of Working Solution
Corresponding to the BrdU-labeling reagent which is included in the Roche Cell Proliferation ELISA, BrdU kits and in the Roche 5-Bromo-2′-deoxy-uridine Labeling and Detection Kits, prepare the BrdU working solution by dissolving the substance in PBS to a 10 mM stock solution (MW of BrdU = 307.1 D). For the in vivo use of BrdU dissolve in PBS; for the in vitro use of BrdU, dissolve in double-distilled water at the same 10 mM concentration.
Storage conditions (working solution): -15 to -25 °C

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H340,H361fd
• Conseils de prudence: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Classification des risques: Muta. 1B - Repr. 2
• Code de la classe de stockage: 13 - Non Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable