10634425001

Octyl β-D-glucopyranoside

non-ionic

• Synonyme(s) : n-Octyl glucoside, OGP
• Formule empirique (notation de Hill) : C₁₄H₂₈O₆
• CAS Number: 29836-26-8
• Poids moléculaire : 292.37
• Beilstein: 84118
• Numéro MDL: MFCD00063288
• Code UNSPSC : 12352200
• ID de substance PubChem : 329748920

Propriétés

• Essai: 99% (GC)
• Poids mol.: micellar avg mol wt 25,000; 292.4
• Conditionnement: pkg of 10 g
• Fabricant/nom de marque: Roche
• Nombre d'agrégation: 84
• Technique(s): dialysis: suitable
• CMC: 20-25 mM (20-25°C)
• Température de transition: cloud point >100 °C
• Conditions d'expédition: ambient
• Chaîne SMILES: CCCCCCCCO[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O
• InChI: 1S/C14H28O6/c1-2-3-4-5-6-7-8-19-14-13(18)12(17)11(16)10(9-15)20-14/h10-18H,2-9H2,1H3/t10-,11-,12+,13-,14-/m1/s1
• Clé InChI: HEGSGKPQLMEBJL-RKQHYHRCSA-N

Description

Description générale

1-O-n-Octyl-β-D-glucopyranoside, octylglucoside, is a detergent with an octyl chain as hydrophobic residue and glucose as hydrophilic part. It is a non-ionic, dialyzable, mild and non-denaturing detergent which is used for the solubilization, isolation and reconstitution of membrane proteins.

Application

Détergent non ionique, dialysable, pour la solubilisation et l′isolement des protéines membranaires. Permet d′augmenter la résolution des protéines dans les gels 2D.

N-Octylglucoside has been shown to increase the resolution of proteins in 2D gels. It is a mild and non-denaturing detergent for the solubilization and reconstitution of membrane proteins. N-Octylglucoside is easily removed by dialysis. N-Octylglucoside has been used in mass spectrometric immunoassay to homogenize MALDI (matrix assisted laser desorption/ionization) matrix draw and elution.

Qualité

Purity: 99% (from C), homogeneous (GC)

Ease of removal*: ++
CMC**: 14.5 mM at +25°C
Formula: C₁₄H₂₈O₆

Notes préparatoires

Working concentration: 46 mM
Working solution: Solubility: > 50% (w/v) in double-dist. water, Tris-HCl 0.05 mol/l; pH7.4 or K-phosphate buffer 0.1 mol/l, pH 7.0 at 25 °C.
Storage conditions (working solution): Stability in Solution
A stock solution is stable at 2 to 8 °C for approx. 3 days. We would expect a long term stability of 6-12 months if stored frozen in portions at -15 to -25 °C, provided bacterial contamination is avoided.

Stockage et stabilité

Store at 15 to 25 °C. (Store dry!)

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable