10814270001

Primer for cDNA Synthesis

lyophilized, pkg of 40 μg

• Synonyme(s) : oligo-p(dt)₁₅
• Code UNSPSC : 41106300

Propriétés

• Forme: lyophilized
• Niveau de qualité: 100
• Conditionnement: pkg of 40 μg
• Fabricant/nom de marque: Roche
• Température de stockage: −20°C (−15°C to −25°C)

Description

Description générale

This primer consists of a 15-mer oligonucleotide that shows thymidine (T) at each individual position. The oligonucleotides are HPLC purified, desalted, and supplied as a lyophilizate of the Li-salt. The p(dT)₁₅ primer is not phosphorylated at the 5′ end.Oligo(dT)N generates full-length cDNAs. If template contains oligo(A) stretches internally, the primer may bind these, causing mis priming and transcripts that are not full-length.

Composition: p(dT)₁₅[ 15-mer oligonucleotide containing only deoxythymidine (dT) residues].

Phosphorylation: The p(dT)₁₅ primer is not phosphorylated at the 5′ end.

Application

Primer for cDNA synthesis may be used in:

• RT-PCR
• Generation of cDNA libraries
• Synthesis of first-strand cDNA

Définition de l'unité

8 nmol ξ 1 A₂₆₀ unit

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): does not flash
• Point d'éclair (°C): does not flash