11119915001

RNase, DNase-free

from bovine pancreas

• Synonyme(s) : Rnase
• Code UNSPSC : 41105600

Propriétés

• Source biologique: bovine pancreas
• Niveau de qualité: 100
• Forme: solution
• Activité spécifique: ≥30 units/mg protein
• Conditionnement: pkg of 500 μg (1 ml)
• Fabricant/nom de marque: Roche
• Technique(s): DNA purification: suitable
• Température de stockage: −20°C

Description

Description générale

Pyrimidine-specific endoribonuclease that acts on single-stranded RNA. RNase, DNase-free, is a heterogeneous mixture of ribonucleases that has been prepared free of deoxyribonuclease activity according to the current Quality Control procedures. RNase, DNase-free, is particularly well suited for use in DNA isolation procedures. Before use, most RNase preparations must be boiled to remove DNase activity. This preparation of RNase does not need to be boiled; it can be used directly from the vial.

Application

RNase, DNase-free, efficiently removes contaminating RNA from plasmid or genomic DNA preparations.

Définition de l'unité

One Kunitz unit is the amount of enzyme that causes a decrease in absorbance of A₀ to A₁ within one minute under the assay conditions. A₀ to A₁ corresponds to the total conversion, A₁ being the final absorbance.
One unit produces a decrease in absorbance at 260 nm, which is equivalent to a total conversion of RNA to oligonucleotides in one minute at +25 °C.

Forme physique

Solution, 500 μg/ml, in 10 mM Tris-HCl, 5 mM CaCl₂, 50% glycerol (pH 7.0).

Notes préparatoires

Working concentration: The optimal working concentration for RNase, DNase free, is 2 to 5 μg/ml. The reaction volume will vary for different applications. Some suggested guidelines are given below:

1. For small-scale isolation of plasmid DNA ("miniprep" from a 1.5 ml bacterial culture), use 0.5 μl of RNase, DNase-free in a reaction volume of 50 μl.
2. To isolate plasmid DNA from a 100 ml bacterial culture, use 8 μl of RNase, DNase-free in a reaction volume of 2 ml.
3. To isolate genomic DNA from cultured mammalian cells (5 x 10⁷ cells), use 8 μl of RNase, DNase-free in a reaction volume of 2 ml.

Working solution: Storage and Dilution Buffer: 10 mM Tris-HCl, 5 mM CaCl₂, 50% glycerol (v/v), pH 7.0.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): No data available
• Point d'éclair (°C): No data available