50484

Methylene Blue solution

suitable for microbiology

• Synonyme(s) : Loeffler’s Methylene Blue
• Formule empirique (notation de Hill) : C₁₆H₁₈ClN₃S
• CAS Number: 61-73-4
• Poids moléculaire : 319.85
• Beilstein: 3641570
• Numéro MDL: MFCD00012111
• Code UNSPSC : 12171500
• ID de substance PubChem : 329757603
• Nomenclature NACRES : NA.47

Propriétés

• Durée de conservation: limited shelf life, expiry date on the label
• Concentration: 0.5% in H₂O (0.5 g/100 mL)
• Technique(s): microbe id | staining: suitable
• Application(s): microbiology
• Adéquation: Bacillus spp.; Corynebacterium spp.; bacteria
• Chaîne SMILES: [Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C
• InChI: 1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1
• Clé InChI: CXKWCBBOMKCUKX-UHFFFAOYSA-M

Description

Description générale

Methylene Blue was earlier recognized as a good nuclear stain, the staining solutions are always either aged or alkalized, if not both. More importantly it is an excellent stain for ribonucleic acid, which is the important stainable constituent of bacterial bodies, of lymphocyte and hemoportoal cytoplasm, and of nerve cell tigroid granules. It can also be employed in capsule stain. The capsule stain begins as a negative stain leaving the organisms colorless. The capsule are clearly seen as halo surrounding the bacterial cell. Another staining procedure is performed with nigrosin (Cat. No. 43925).

Composants

reagent contains:
methylene blue 0.5 g
distilled water 100 ml

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves