60983

Kovac′s reagent for indoles

suitable for microbiology

• Synonyme(s) : 4-(Dimethylamino)benzaldehyde solution, Indole reagent according to Kovac
• Formule empirique (notation de Hill) : C₉H₁₁NO
• CAS Number: 100-10-7
• Poids moléculaire : 149.19
• Beilstein: 4132845
• Numéro MDL: MFCD00003381
• Code UNSPSC : 41171621
• ID de substance PubChem : 329759335
• Nomenclature NACRES : NA.85

Propriétés

• Durée de conservation: limited shelf life, expiry date on the label
• Niveau de qualité: 100
• Composition: n-butanol ; 4-dimethylaminobenzaldehyde ; hydrochloric acid
• Technique(s): microbe id | specific enzyme detection: suitable
• Indice de réfraction: n20/D 1.400-1.420
• Application(s): agriculture; clinical testing; environmental; food and beverages; microbiology
• Température de stockage: 2-8°C
• Adéquation: Escherichia coli; coliforms
• Chaîne SMILES: [H]C(=O)c1ccc(cc1)N(C)C
• InChI: 1S/C9H11NO/c1-10(2)9-5-3-8(7-11)4-6-9/h3-7H,1-2H3
• Clé InChI: BGNGWHSBYQYVRX-UHFFFAOYSA-N

Description

Description générale

Kovac′s reagent for indoles is a solution of 4-(dimethylamino)benzaldehyde and hydrochloric acid in n-butanol.

Application

Kovac′s reagent for indoles has been used for enumerating E. coli O157:H7 in ground beef samples in different scenarios of stored, thawed, and as cooked patty samples. It has also been used for E coli enumeration in dewatered blackwater.

Actions biochimiques/physiologiques

Kovac′s reagent detects the indole and aids in identifying the indole-positive and indole-negative microorganisms. E. coli can split tryptophan into indole and α-aminopropionic acid in the presence of the enzyme tryptophanase. p-Aminobenzaldehyde present in the reagent reacts with indole to form a cherry-red complex, that is soluble in alcohol, ether, and chloroform. It is recommended to use glucose free media and peptone with a high tryptophan content for the test.

Informations sur la sécurité

• Pictogrammes: GHS02,GHS05,GHS07
• Mention d'avertissement: Danger
• Mentions de danger: H226,H290,H302,H315,H318,H335,H336
• Conseils de prudence: P210 - P233 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
• Classification des risques: Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Central nervous system, Respiratory system
• Code de la classe de stockage: 3 - Flammable liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): 96.8 °F
• Point d'éclair (°C): 36 °C
• Équipement de protection individuelle: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter