63069

Chlorure de magnésium solution

BioUltra, Molecular Biology, ~1 M in H₂O

• Synonyme(s) : Magnesium Chloride hydrate, Magnesium chloride (MgCl₂)hexahydrate, MgCl₂
• Formule linéaire : MgCl₂
• CAS Number: 7786-30-3
• Poids moléculaire : 95.21
• Numéro MDL: MFCD00011106
• Code UNSPSC : 12352302
• ID de substance PubChem : 329759772
• Nomenclature NACRES : NA.26

Propriétés

• Qualité: Molecular Biology
• Niveau de qualité: 200
• Gamme de produits: BioUltra
• Forme: liquid
• Concentration: ~1 M in H₂O
• Technique(s): HPLC: suitable; flow cytometry: suitable
• Impuretés: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected; ≤0.0002% total Nitrogen (N)
• pH: 5.0-7.5 (25 °C, 1 M in H₂O)
• Solubilité: water: soluble
• Densité: 1.08 g/mL at 20 °C
• Traces d'anions: phosphate (PO₄^3-): ≤10 mg/kg; sulfate (SO₄^2-): ≤50 mg/kg
• Traces de cations: Al: ≤1 mg/kg; As: ≤0.1 mg/kg; Ba: ≤1 mg/kg; Bi: ≤1 mg/kg; Ca: ≤10 mg/kg; Cd: ≤1 mg/kg; Co: ≤1 mg/kg; Cr: ≤1 mg/kg; Cu: ≤1 mg/kg; Fe: ≤1 mg/kg; K: ≤50 mg/kg; Li: ≤1 mg/kg; Mn: ≤1 mg/kg; Mo: ≤1 mg/kg; Na: ≤500 mg/kg; Ni: ≤1 mg/kg; Pb: ≤1 mg/kg; Sr: ≤1 mg/kg; Zn: ≤1 mg/kg
• λ: 1 M in H₂O
• Absorption UV: λ: 260 nm A_max: 0.05; λ: 280 nm A_max: 0.03
• Adéquation: suitable for molecular biology
• Chaîne SMILES: Cl[Mg]Cl
• Application(s): cell analysis; filtration; vaccine development
• InChI: 1S/2ClH.Mg/h2*1H;/q;;+2/p-2
• Clé InChI: TWRXJAOTZQYOKJ-UHFFFAOYSA-L

Description

Description générale

Magnesium chloride is widely used in polymerase chain reactions (PCR) buffers to supply magnesium ions. The concentration of Mg²⁺ is the most critical parameter that impacts both the efficiency and specificity of PCR when total genomic DNA is used, synthesis of long products, or during multiplex reactions. Elevated concentrations of Mg²⁺ enhance the stability of primer-template duplexes and the efficiency of amplification.

Application

Magnesium chloride solution has been used as a component of nuclei isolation buffer for in situ fixations and isolation of nuclei from Kupffer cells (or other liver cells).

Magnesium chloride solution has been used in the reaction mix of reverse transcriptase enzyme, PFV RT. It has been used in the preparation of artificial cerebrospinal fluid (ACSF).

Autres remarques

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Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves