93321

TRIS Glycine buffer solution

BioUltra, 10× concentrate

• Synonyme(s) : Blotting buffer solution
• Code UNSPSC : 41105319
• Nomenclature NACRES : NA.25

Propriétés

• Gamme de produits: BioUltra
• Niveau de qualité: 100
• Forme: liquid
• Qualité: 10× concentrate
• Composition: glycine, 1.92 M ; TRIS, 0.25 M
• Impuretés: insoluble matter, passes filter test; proteases, none detected
• Résidus de calcination: ≤0.2%
• pH: 8.5-8.7
• Traces d'anions: sulfate (SO₄^2-): ≤50 mg/kg
• Traces de cations: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; NH₄⁺: ≤200 mg/kg; Na: ≤500 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• λ: neat
• Absorption UV: λ: 260 nm A_max: 0.1; λ: 280 nm A_max: 0.1

Description

Application

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. TRIS Glycine buffer solution, 10X concentrate is diluted to a working concentration of 1X and pH adjusted as required. TRIS Glycine buffer (TGB) is frequently used in gel electrophoresis and ion-exchange chromatography applications.

Autres remarques

Electrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves