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Middlebrook 7H10 Agar Base

suitable for microbiology, NutriSelect® Plus, for use in isolation and cultivation of Mycobacterium species

Synonyme(s) :

Mycobacterial culture media

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100 EA
CA$497.00

CA$497.00


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100 EA
CA$497.00

About This Item

Code UNSPSC :
41171609
Nomenclature NACRES :
NA.85

CA$497.00


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Niveau de qualité

Stérilité

non-sterile

Gamme de produits

BioChemika

Forme

powder

Durée de conservation

limited shelf life, expiry date on the label

Conditionnement

pkg of 500 g

Fabricant/nom de marque

NutriSelect® Plus

Technique(s)

microbe id | susceptibility testing: suitable
microbiological culture: suitable

pH final

6.6±0.2 (25 °C)

Application(s)

clinical testing
food and beverages
life science and biopharma
veterinary

microbiology

Catégories apparentées

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Cet article
CLS3738CLS3736CLS3527
binding type

non-treated surface

binding type

non-treated surface

binding type

non-treated surface

binding type

Tissue Culture (TC)-treated surface

sterility

sterile; γ-irradiated by SER-TAIN process

sterility

sterile; γ-irradiated by SER-TAIN process

sterility

sterile; γ-irradiated by SER-TAIN process

sterility

sterile

manufacturer/tradename

Corning 3737

manufacturer/tradename

Corning 3738

manufacturer/tradename

Corning 3736

manufacturer/tradename

Corning 3527

material

clear , flat bottom , clear bottom, polystyrene

material

clear , clear bottom, flat bottom , polystyrene

material

clear , clear bottom, flat bottom , polystyrene

material

clear bottom, flat bottom wells, polystyrene plate, round clear wells

feature

lid, skirt

feature

lid, skirt

feature

lid, skirt

feature

lid, skirt

suitability

suitable for (cell culture)

suitability

suitable for (cell culture)

suitability

suitable for (cell culture)

suitability

-

Description générale

Middlebrook 7H10 Agar Base is recommended for isolation, cultivation and sensitivity testing of Mycobacterium tuberculosis. Middlebrook media consists of many inorganic salts, which help, in growth of Mycobacteria. Citric acid formed from sodium citrate helps in retaining inorganic cations in solution. Glycerol supplies carbon and energy. Middlebrook OADC Growth Supplement (M0678) contains oleic acid, bovine albumin, sodium chloride, dextrose and catalase. Oleic acid and other long chain fatty acids are essential for metabolism of Mycobacteria. Some free fatty acids are toxic to Mycobacteria, but albumin binds to those fatty acids and prevents toxic action on Mycobacteria. Dextrose serves as an energy source. Catalase neutralizes toxic peroxides. Malachite green partially inhibits other bacteria.

Application

Middlebrook 7H10 Agar Base is a selective media for isolation, cultivation and sensitivity testing of Mycobacterium tuberculosis. Mycobacteria grow most rapidly in broth media and hence primary isolation of bacterial species can be carried out. It is inexpensive, easy to prepare and can aid in early detection of acid-fast, Mycobacterium species by culture.

Composants

Ingredients (g/L)
Ammonium sulfate, 0.50
L-Glutamic acid, 0.50
Monopotassium phosphate, 1.50
Disodium phosphate, 1.50
Sodium citrate, 0.40
Ferric ammonium citrate, 0.04
Magnesium sulfate, 0.025
Calcium chloride, 0.0005
Zinc sulfate, 0.001
Copper sulfate, 0.001
Pyridoxine hydrochloride, 0.001
Biotin, 0.0005
Malachite green, 0.00025
Agar, 15.00

Notes préparatoires

Suspend 19.47 g of Middlebrook 7H10 Agar Base in 900 ml of distilled water. Add 5 ml of glycerol (Cat. No. 49767). Boil to dissolve the medium completely. Distribute in 180 ml amounts in erlenmeyer flasks and sterilize by autoclaving at 15 lbs. pressure (121°C) for 10 minutes. Cool to 45°C and aseptically add 20 ml of Middlebrook OADC Growth Supplement (Cat. No. M0678). Mix well and pour into screw capped tubes.Note: Keep prepared medium in the dark before and after inoculation.

Note de bas de page

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Informations légales

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Chuanjin Yu et al.
Journal of integrative plant biology, 62(4), 509-526 (2019-02-26)
Trichoderma harzianum is a plant-beneficial fungus that secretes small cysteine-rich proteins that induce plant defense responses; however, the molecular mechanism involved in this induction is largely unknown. Here, we report that the class II hydrophobin ThHyd1 acts as an elicitor

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