46950

Fluorescein 5(6)-isothiocyanate

BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)

• Synonyme(s) : FITC, Fluorescein isothiocyanate
• Formule empirique (notation de Hill) : C₂₁H₁₁NO₅S
• CAS Number: 27072-45-3
• Poids moléculaire : 389.38
• Beilstein: 1407295
• Numéro CE : 248-207-7
• Numéro MDL: MFCD00005063
• Code UNSPSC : 12171500
• ID de substance PubChem : 329757273
• Nomenclature NACRES : NA.32

Propriétés

• Niveau de qualité: 100
• Gamme de produits: BioReagent
• Essai: ≥90% (HPLC)
• Fluorescence: λ_ex 492 nm; λ_em 518 nm in 0.1 M phosphate pH 8.0
• Adéquation: suitable for fluorescence
• Température de stockage: −20°C
• Chaîne SMILES: Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
• InChI: 1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
• Clé InChI: MHMNJMPURVTYEJ-UHFFFAOYSA-N

Description

Description générale

Fluorescein 5(6)-isothiocyanate, also known as FITC, is a weakly acidic hydroxyxanthene whose overall size is small, with both carboxylic acid and isothiocyanate substituents on the pendant phenyl ring. The dye has a moderately sized, nonplanar aromatic system. The isothiocyanate group reacts with nucleophilic substituents (e.g. amino, hydroxyl, thiol) of biomolecules to be attached as a fluorescent label. The maximum emission of the dye is at 519nm in the same solvent.

Application

Fluorescein 5(6)-isothiocyanate (FITC) labelled molecules are used in non-microscopic immunoassays and physiological tracing experiments. FITC is widely used in flow cytometry experiments for staining proteins, nuclei, and subnuclear particles. White blood cells (WBCs) are marked with FITC to study their response toward a chemoattractant, eventually being measured.

For fluorescent labeling of proteins (e.g. immunofluorescence); H Protein labeling for gel chromatography

Conditionnement

Bottomless glass bottle. Contents are inside inserted fused cone.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Faceshields, Gloves