50983

Guanidine thiocyanate solution

BioUltra, ~6 M in H₂O

• Synonyme(s) : Guanidine isothiocyanate, Guanidinium isothiocyanate, Guanidinium rhodanide, Guanidinium thiocyanate
• Formule linéaire : NH₂C(=NH)NH₂ · HSCN
• CAS Number: 593-84-0
• Poids moléculaire : 118.16
• Beilstein: 3563461
• Numéro MDL: MFCD00013027
• Code UNSPSC : 12352107
• ID de substance PubChem : 57651292
• Nomenclature NACRES : NA.25

Propriétés

• Qualité: Molecular Biology
• Niveau de qualité: 200
• Gamme de produits: BioUltra
• Concentration: ~6 M in H₂O
• Impuretés: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• Indice de réfraction: n20/D 1.474-1.483
• pH: 4.5-7.0 (25 °C)
• Traces d'anions: chloride (Cl^-): ≤500 mg/kg; sulfate (SO₄^2-): ≤50 mg/kg
• Traces de cations: Al: ≤1 mg/kg; As: ≤0.1 mg/kg; Ba: ≤1 mg/kg; Bi: ≤1 mg/kg; Ca: ≤5 mg/kg; Cd: ≤1 mg/kg; Co: ≤1 mg/kg; Cr: ≤1 mg/kg; Cu: ≤1 mg/kg; Fe: ≤1 mg/kg; K: ≤20 mg/kg; Li: ≤1 mg/kg; Mg: ≤1 mg/kg; Mn: ≤1 mg/kg; Mo: ≤1 mg/kg; Na: ≤500 mg/kg; Ni: ≤1 mg/kg; Pb: ≤1 mg/kg; Sr: ≤1 mg/kg; Zn: ≤1 mg/kg
• Absorption: cut-off at 280 nm
• Chaîne SMILES: SC#N.NC(N)=N
• InChI: 1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H
• Clé InChI: ZJYYHGLJYGJLLN-UHFFFAOYSA-N

Description

Description générale

Guanidine thiocyanate is a chaotropic agent that functions as a strong protein denaturant. It is widely used in RNA isolation protocols. Guanidine thiocyanate can irreversibly inactivate RNase and DNase and breaks cellular membranes. It dissolves proteins by disturbing their secondary structures. Guanidine thiocyanate also separates nucleoproteins from nucleic acids and aids the digestion of the proteins with proteases. It improves the activity of proteinase K thus making it the protease of choice.

Application

Guanidine thiocyanate solution has been used to extract:

• plasma RNA from human immunodeficiency virus (HIV)-infected and control patients
• total RNA from collected tissues (shoots, flowers, fruits, and seeds)
• in reverse transcription-polymerase chain reaction (RT-PCR) analysis, RNA from teratocarcinoma cell line (Tera-1)

Attention

possibly crystallized product can be dissolved again by slightly heating to 40-50 °C.

Informations sur la sécurité

• Pictogrammes: GHS05,GHS07
• Mention d'avertissement: Danger
• Mentions de danger: H302 + H312 + H332,H314,H412
• Conseils de prudence: P273 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Classification des risques: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C
• Risques supp: EUH032
• Code de la classe de stockage: 8A - Combustible corrosive hazardous materials
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter