81349

Poly(2′-deoxyinosinic-2′-deoxycytidylic acid) sodium salt

free from low molecular weight nucleotides, lyophilized

• Synonyme(s) : Poly(dI-dC) sodium salt
• CAS Number: 118578-37-3
• Numéro MDL: MFCD00147947
• Code UNSPSC : 12352106
• Nomenclature NACRES : NA.52

Propriétés

• Qualité: Molecular Biology; lyophilized
• Niveau de qualité: 100
• Forme: powder
• Poids mol.: M_r ~2.5 × 10⁶ (broad molecular weight distribution); M_r ~2500000
• Conditionnement: ampule of ≥10 units A₂₆₀
• Température de stockage: −20°C

Description

Description générale

Poly (2′-deoxyinosinic-2′-deoxycytidylic acid) sodium salt is an alternating copolymer that acts as a substrate for DNA methyltransferases.

Application

Poly(2′-deoxyinosinic-2′-deoxycytidylic acid) sodium salt has been used in electrophoretic mobility shift assay (EMSA) of the transcription factor AP-2 Alpha (TFAP2A) and in southwestern blotting of human embryonic kidney cells (HEK293) nuclear extract.

Suitable for

• non-specific blocking agent in EMSA
• evaluation of DNA methyltransferases
• DNA-small molecule or drug interaction studies

Actions biochimiques/physiologiques

Poly(2′-deoxyinosinic-2′-deoxycytidylic acid), poly(dI-dC) is a synthetic DNA substrate. At high salt, Poly(dI-dC) exists in left handed helical conformation and reverts to right-handed form upon decreasing salt. It is used as a non-specific competitor in electrophoretic mobility shift assay (EMSA).

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)