A2289

Albumin, Tetramethylrhodamine isothiocyanate bovine

lyophilized powder

• Synonyme(s) : TRITC-albumin
• CAS Number: 9048-46-8
• Numéro MDL: MFCD00130416
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.25

Propriétés

• Source biologique: bovine
• Essai: ≥90% (Lowry)
• Forme: lyophilized powder
• Concentration: ≥90% protein (Lowry)
• Ampleur du marquage: ≥0.5 mol TRITC per mol albumin
• Technique(s): cell culture | mammalian: suitable
• Impuretés: salt, free
• Numéro d'accès UniProt: P02769
• Température de stockage: 2-8°C
• Informations sur le gène: bovine ... ALB(280717)

Description

Description générale

Tetramethylrhodamine isothiocyanate (TRITC) is stable and less sensitive to light compared to fluorescein isothiocyanate (FITC). The conjugate of albumin with TRITC is effective for following microcirculation in vivo microscopy. Albumin-TRITC aids tracking of calcium phosphate nanoparticle bound DNA in human umbilical vein endothelial cells (T-HUVEC).

Application

Albumin, Tetramethylrhodamine isothiocyanate has been used:

• in photoinduced release assay of polyethylene beads
• for passivating poly(methyl methacrylate) (PMMA) layer prior to immunoglobulin G coating
• in fluorescent correlation spectroscopy

Red-fluorescing TRITC-albumin was used to track the uptake of calcium phosphate/DNA nanoparticles during cell transfection. The dye-linked albumin is incorporated into the nanoparticles. Efficiency of transfection could be followed by fluorescence microscopy.

Notes préparatoires

Prepared from crystallized and lyophilized albumin (A4378).

Informations sur la sécurité

• Pictogrammes: GHS08,GHS07
• Mention d'avertissement: Danger
• Mentions de danger: H302 + H312 + H332,H315,H319,H334,H335
• Conseils de prudence: P261 - P264 - P280 - P301 + P312 - P302 + P352 + P312 - P305 + P351 + P338
• Classification des risques: Acute Tox. 4 Oral - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 13 - Non Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Faceshields, Gloves