A7080

Amphiregulin human

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonyme(s) : AREG, CRDGF, SDGF, AR
• CAS Number: 117147-70-3
• Numéro MDL: MFCD00282118
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• Product Name: Amphiregulin human, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture, ≥97% (SDS-PAGE)
• Source biologique: human
• Niveau de qualité: 100
• Produit recombinant: expressed in E. coli
• Essai: ≥97% (SDS-PAGE)
• Forme: lyophilized powder
• Puissance: 10-100 ng/mL ED₅₀/EC₅₀
• Poids mol.: ~11 kDa
• Conditionnement: pkg of 1 mg
• Conditions de stockage: avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)
• Technique(s): cell culture | mammalian: suitable
• Impuretés: endotoxin, tested
• Numéro d'accès UniProt: P15514
• Température de stockage: −20°C
• Informations sur le gène: human ... AREG(374)

Description

Actions biochimiques/physiologiques

Also known as keratinocyte autocrine factor (KAF); expression of amphiregulin mRNA has been found in numerous carcinoma cell lines and epithelial cells of several human tissues including colon, stomach, breast, ovary, and kidney.

Composants

Human amphiregulin is produced by a DNA sequence that encodes a 98 amino acid residue form of mature human amphiregulin. This corresponds to amino acid residues 101-198 in E. coli. Amphiregulin is a glycoprotein produced in response to treatment of the cell line MCF-7, a human breast carcinoma cell line, with PMA, a protein kinase C activator and potent tumor promoter.

Attention

This product should be stored at -20°C. After preparation, the product can be stored at 2-8°C for one month, or frozen in aliquots at -20°C or -70°C for more extended storage.

Forme physique

Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4 with 50 μg BSA per 1 μg as a carrier protein.

Notes préparatoires

It should be reconstituted by adding sterile PBS containing 0.1% HAS or BSA to a concentration greater than 10 μg/mL.

Remarque sur l'analyse

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Marquardt, H. et al. (1984) Science 223:1079. The ED₅₀ for this effect is typically 5-15 ng/mL.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)