A7089

Accumax^™ solution

sterile-filtered, suitable for cell culture

• Synonyme(s) : Cell detachment solution
• Numéro MDL: MFCD01632904
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.75

Propriétés

• Stérilité: sterile-filtered
• Niveau de qualité: 200
• Forme: solution
• Concentration: 1 ×
• Technique(s): cell culture | mammalian: suitable; single cell analysis: suitable
• pH: 6.8-7.8
• Conditions d'expédition: dry ice
• Température de stockage: −20°C

Description

Application

Accumax has proven effective in dissociating clumped cells in suspension cultures of hybridomas, CHO, BHK, 293, COS and Sf9 cells. The solution performed extremely well in dissociating cell counting, viral transfection assays, cell sorting, flow cytometry, and bioreactor scale-up. It has also been used to create single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue, and the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware.

TM=["Accumax"] solution has been used:

• for detaching single walled carbon nanotubes from the extracellular matrix (ECM) in epithelial cells
• for dissociation of retinal pigmented epithelial cells prior to passaging
• for dissociation of islets from pancreas

Actions biochimiques/physiologiques

Accumax is a cell detachment solution consisting of proteolytic and collagenolytic enzymes. Each bottle has 100 mL of 1x Accumax enzymes in Dulbecco′s PBS. There are no mammalian or bacterial-derived products found in Accumax.

Caractéristiques et avantages

Ready-to-use sterile liquid for in vitro cell applications

Attention

Accumax is stable when stored at -20°C. After thawing, the product can be stored for up to 2 months at 4°C.

Variante de formule

Prepared in Dulbecco′s PBS (0.2 g/L KCl, 0.2 g/L KH₂PO₄, 8 g/L NaCl, and 1.15 g/L Na₂HPO₄). Refer to Product Data Sheet on the website for product usage information.

Notes préparatoires

This product is supplied as a sterile, ready-to-use frozen liquid. Accumax should be thawed at 37°C before use, and added at equal volume to a sample of clumped cells. Allow 5-10 minutes at 37°C for incubation and count cells as you would normally.

Informations légales

Accumax is a trademark of Innovative Cell Technologies, Inc.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable