A7510

Adenosine 5′-Triphosphatase from porcine cerebral cortex

lyophilized powder, ≥0.3 units/mg protein, pH 7.8

• Synonyme(s) : ATP phosphohydrolase, ATPase
• CAS Number: 9000-83-3
• Numéro CE : 3.6.1.3 (BRENDA, IUBMB)
• Numéro MDL: MFCD00135671
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: Porcine cerebral cortex
• Niveau de qualité: 200
• Description: Sodium and Potassium-Activated
• Forme: lyophilized powder
• Activité spécifique: ≥0.3 units/mg protein, pH 7.8
• Composition: Protein, 5.0-25.0% biuret
• Technique(s): inhibition assay: suitable
• Numéro d'accès UniProt: P05024; P05027
• Température de stockage: −20°C
• Informations sur le gène: pig ... ATP1A1(397481), ATP1B1(396898)

Description

Application

ATPase is used to liberate inorganic phosphorus from ATP. ATPase, from porcine cerebral cortex, has been used in inhibition assays of Na/K-ATPase activity .

Actions biochimiques/physiologiques

This membrane enzyme hydrolyzes ATP to ADP and orthophosphate. The hydrolysis is coupled with the exchange of sodium and potassium ions across the plasma membrane. The enzyme is present in almost all tissues of higher organisms, but it is most abundant in the kidney where it reabsorbs sodium ions from the glomerular filtrate. Na⁺,K⁺-ATPase is affected by changes in intracellular calcium. It is also sensitive to ouabain and inhibited by vanadate, and (in vitro) by SPAI-1, a peptide isolated from porcine duodenum. It has been reported to be regulated by phosphorylation by protein kinase A (PKA).

This membrane enzyme hydrolyzes ATP to ADP and orthophosphate. The hydrolysis is coupled with the exchange of sodium and potassium ions across the plasma membrane. The enzyme is present in almost all tissues of higher organisms, but it is most abundant in the kidney where it reabsorbs sodium ions from the glomerular filtrate. Na+,K+-ATPase is affected by changes in intracellular calcium. It is also sensitive to ouabain and inhibited by vanadate, and (in vitro) by SPAI-1, a peptide isolated from porcine duodenum. It has been reported to be regulated by phosphorylation by protein kinase A (PKA). Bufalin, and other cardiotonic steroids, may inhibit Na+/K+-ATPase activity and induce the differentiation of HL60, U937, and ML1 cells .

Définition de l'unité

One unit will liberate 1.0 μmole of inorganic phosphorus from ATP per min at pH 7.8 at 37 °C in the presence of Na⁺, K^+, and Mg²⁺.

Forme physique

Lyophilized powder containing 90% sucrose, 0.4% EDTA Na₄ and 0.06% NaCl

Notes préparatoires

Sodium iodide extracted fraction of Nakao, et al., Biochem. Biophys. Res. Commun., 19, 755 (1965).

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)