B2787

Biotinylated Molecular Weight Marker

mol wt 6,500-180,000 Da

• Synonyme(s) : protein markers, protein molecular weight markers, protein standards
• Code UNSPSC : 12352200
• eCl@ss : 34058011
• Nomenclature NACRES : NA.25

Propriétés

• Forme: lyophilized powder
• Niveau de qualité: 200
• Utilisation: 1 vial sufficient for 200 applications (on 10 cm × 10 cm mini gels)
• Poids mol.: 6,500-180,000 Da
• Conditionnement: vial of 0.1 mg protein (also contains 0.1 mg NaCl and 0.1 mg sucrose)
• Température de stockage: −20°C

Description

Application

For SDS-PAGE and protein transfer, the Biotinylated Molecular Weight Standard Mixture contains proteins, which have been biotin conjugated for use as SDS-PAGE standards for the estimation of the molecular weights of blotted proteins. Visualization is accomplished using streptavidin-peroxidase (Cat. No. S2438) and a color development reagent such as 4-Chloro-1-naphthol (Cat. No. C8890), eliminating the need to cut out separate lanes for general protein stains and can be performed simultaneously with immunostaining procedures. These standards may be used with either the Laemmli or the Weber and Osborn SDS-PAGE procedures.

Composants

This product contains:

• α₂-macroglobulin (equine serum)
• β-galactosidase (E. coli)
• phosphorylase b (rabbit muscle)
• catalase (bovine liver)
• alcohol dehydrogenase (yeast)
• carbonic anhydrase (bovine erythrocyte)
• trypsin inhibitor (soy bean)
• lysozyme (chicken egg)
• aprotinin (bovine lung)

Each vial contains approximately 33% protein, 33% NaCl and 33% sucrose.

Autres remarques

Contains nine biotinylated proteins (approx 0.1 mg total).

Reconstitution

Each vial is reconstituted with 1 mL of 1x sample buffer (Cat. No. S3401) and incubated for 2-3 minutes at 100 °C.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable