B3795

Brain-derived neurotrophic factor human

≥95% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonyme(s) : Abrineurin, BDNF
• Numéro MDL: MFCD00285579
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• Product Name: Brain-derived neurotrophic factor human, BDNF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• Source biologique: human
• Niveau de qualité: 200
• Produit recombinant: expressed in E. coli
• Essai: ≥95% (SDS-PAGE)
• Forme: lyophilized powder
• Qualité: endotoxin tested
• Poids mol.: ~27 kDa
• Conditionnement: pkg of 10 μg; pkg of 5 μg
• Conditions de stockage: avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)
• Technique(s): cell culture | mammalian: suitable
• Impuretés: ≤1.00 EU/μg Endotoxin level
• Numéro d'accès UniProt: P23560
• Température de stockage: −20°C
• Informations sur le gène: human ... BDNF(627), BDNF(627)

Description

Description générale

BDNF is a member of the neurotrophin family of growth factors that includes NGF, NT-3, and NT-4. All neurotrophins have six conserved cysteine residues and share a 55% sequence identity at the amino acid level. BDNF has been shown to enhance the survival and differentiation of several classes of neurons in vitro, including neural crest and placode-derived sensory neurons, dopaminergic neurons in the substantia nigra, basal forebrain cholinergic neurons, hippocampal neurons, and retinal ganglial cells.1 BDNF is expressed within peripheral ganglia and is not restricted to neuronal target fields, raising the possibility that BDNF has paracrine or even autocrine actions on neurons as well as non-neuronal cells.
The active form of BDNF recombinant human protein (27 kDa) is a dimer formed by two identical 119 amino acid subunits held together by strong hydrophobic interactions

Application

Brain-derived neurotrophic factor (BDNF) recombinant human protein has been used as a supplement in various types of culture media.

Actions biochimiques/physiologiques

Brain-derived neurotrophic factor (BDNF) is a 13.6 kDa (or 27.2 kDa dimer) member of the neurotrophin family. BDNF has identical amino acid sequence in human, mouse, and pig with full cross-reactivities. BDNF is important in development and maintenance of neuronal populations within the central nervous system or cells directly associated with it. BDNF has been shown to enhance the survival and differentiation of several classes of neurons in vitro, including neural crest and placode-derived sensory neurons, dopaminergic neurons in the substantia nigra, basal forebrain cholinergic neurons, hippocampal neurons, and retinal ganglial cells.

Forme physique

lyophilized from a sterile 0.2 μm filtered solution with BSA

Remarque sur l'analyse

The biological activity is determined by its ability to stimulate the proliferation of human neuroblastoma cells, SH-SY5Y.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)