B3928
Blue-White Select™ Screening Reagent
for selection of recombinant bacterial clones
Synonyme(s) :
Blue-White Reagent, Screening Reagent, Selection Reagent
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About This Item
Code UNSPSC :
12352200
Nomenclature NACRES :
NA.85
Produits recommandés
Qualité
Molecular Biology
Niveau de qualité
Forme
liquid
Utilisation
sufficient for 125 dishes (90 mm)
Technique(s)
nucleic acid detection: suitable
Adéquation
suitable for nucleic acid staining
Conditions d'expédition
dry ice
Température de stockage
−20°C
Description générale
Sigma′s Blue-White Select™ Screening Reagent is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Blue-White Select™ Screening Reagent is designed for blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
Caractéristiques et avantages
- Intense color contrast for easy colony selection
- Convenient, ready-to-use solution
Composants
40 mg/mL IPTG
40 mg/mL X-Gal
(prepared in DMSO)
40 mg/mL X-Gal
(prepared in DMSO)
Principe
When Blue-White Select™ Screening Reagent is cleaved by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of Blue-White Select™ Screening Reagent remain white in color, allowing for easy differentiation between lac+ and lac- colonies.
The technique is based on vectors such as the pUC and the M13mp series that carry a fragment of the β-galactosidase gene encoding an a-fragment of β-galactosidase. Exploitation of these vectors requires use of a bacteria strain carrying the complementing gene fragment to allow the assembly of an active complex, resulting in the formation of blue colonies. Disruption of the ß-galactosidase gene by insertion of a DNA fragment into the vector′s multiple cloning site results in the loss of functional β-galactosidase activity; these colonies remain white, allowing for easy differentiation between lac+ and lac- colonies.
The technique is based on vectors such as the pUC and the M13mp series that carry a fragment of the β-galactosidase gene encoding an a-fragment of β-galactosidase. Exploitation of these vectors requires use of a bacteria strain carrying the complementing gene fragment to allow the assembly of an active complex, resulting in the formation of blue colonies. Disruption of the ß-galactosidase gene by insertion of a DNA fragment into the vector′s multiple cloning site results in the loss of functional β-galactosidase activity; these colonies remain white, allowing for easy differentiation between lac+ and lac- colonies.
Informations légales
Blue-White Select is a trademark of Sigma-Aldrich Co. LLC
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
188.6 °F
Point d'éclair (°C)
87 °C
Équipement de protection individuelle
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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