B4554

BES

BioPerformance Certified, suitable for cell culture, ≥99.0%

• Synonyme(s) : Acide N,N-bis(2-hydroxyéthyl)-2-aminoéthanesulfonique, N,N-Bis(2-hydroxyéthyl)taurine
• Formule empirique (notation de Hill) : C₆H₁₅NO₅S
• CAS Number: 10191-18-1
• Poids moléculaire : 213.25
• Beilstein: 1781572
• Numéro CE : 233-465-5
• Numéro MDL: MFCD00007533
• Code UNSPSC : 12161700
• ID de substance PubChem : 24891788
• Nomenclature NACRES : NA.25

Propriétés

• Qualité: BioPerformance Certified
• Essai: ≥99.0%
• Forme: crystalline
• Technique(s): cell culture | mammalian: suitable
• Impuretés: endotoxin and bioburden, tested
• pH: 2.5-5.0 (25 °C, 213.3 g/L)
• Plage de pH utile: 6.4-7.8
• pKa (25 °C): 7.1
• Solubilité: water: 0.5 g/mL, clear, colorless
• Application(s): diagnostic assay manufacturing
• Activité étrangère: DNAse (exonuclease), NICKase (endonuclease), RNAse, and protease, none detected
• Température de stockage: room temp
• Chaîne SMILES: OCCN(CCO)CCS(O)(=O)=O
• InChI: 1S/C6H15NO5S/c8-4-1-7(2-5-9)3-6-13(10,11)12/h8-9H,1-6H2,(H,10,11,12)
• Clé InChI: AJTVSSFTXWNIRG-UHFFFAOYSA-N

Description

Description générale

N-N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid (BES) is a zwitterionic sodium sulphate buffer used in biochemistry and molecular biology research. BES buffer used as a secondary pH standard can help maintain a stable pH range in solutions, particularly in the range of 6.2-7.6 pH range of 6.6 to 7.4. It ​is used in a study to evaluate that buffers minimize the action of interfering factors in the bacterial endotoxins test. The application of BES as a buffer solution for calcium phosphate-mediated transfection of eukaryotic cells with plasmid DNA has also been studied.

Application

BES buffer has been used:

• as a supplement in opti-minimal essential medium (MEM) culture media to culture thymocytes for In vitro analysis of thymocyte apoptosis
• as a component of borate buffer saline (BBS) for adeno-associated virus (AAV) vector production, extraction, and purification
• as a component of diluent one for semen collection and cryopreservation

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Gloves