B5388

Brilliant Cresyl Blue ALD

Dye content, ≥60%, certified by the Biological Stain Commission, powder

• Synonyme(s) : 3-Amino-7-(diethylamino)-2-methylphenoxazin-5-ium (T-4)-tetrachlorozincate (2:1), Brilliant Blue C, Brilliant Cresyl blue
• Formule linéaire : C₁₇H₂₀N₃OCl · 1/2ZnCl₂
• CAS Number: 51716-96-2
• Poids moléculaire : 385.96
• Numéro C.I. (Colour Index): 51010
• Beilstein: 5690713
• Numéro CE : 279-675-0
• Numéro MDL: MFCD00148901
• Code UNSPSC : 12171500
• ID de substance PubChem : 24891836
• Nomenclature NACRES : NA.47

Propriétés

• Product Name: Brilliant Cresyl Blue ALD, certified by the BSC
• Agence: certified by the BSC
• Niveau de qualité: 200
• Forme: powder
• Composition: Dye content, ≥60%
• Solubilité: water: 1 mg/mL, blue to very deep blue
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: room temp
• Chaîne SMILES: [Zn-2](Cl)(Cl)(Cl)Cl.N(CC)(CC)c4cc5[o+]c6c(nc5cc4)cc(c(c6)N)C.N(CC)(CC)c1cc2[o+]c3c(nc2cc1)cc(c(c3)N)C
• InChI: 1S/2C17H20N3O.4ClH.Zn/c2*1-4-20(5-2)12-6-7-14-16(9-12)21-17-10-13(18)11(3)8-15(17)19-14;;;;;/h2*6-10H,4-5,18H2,1-3H3;4*1H;/q2*+1;;;;;+2/p-4
• Clé InChI: SIHASHFMDFXGSX-UHFFFAOYSA-J

Description

Application

Brilliant Cresyl Blue ALD has been used for the selection of oocytes. It has also been used for the selection of cumulus-oocyte complexes (COCs).

Actions biochimiques/physiologiques

Brilliant Cresyl Blue ALD is mainly used for the selection of oocytes. It helps in determining the activity of glucose-6-phosphate dehydrogenase (G6PD). This enzyme is generated in growing oocytes. However, oocytes which complete their growth have low levels of G6PD. Low G6PD activity results in blue coloration of the cytoplasm because brilliant cresyl blue is not reduced to a colourless compound. It is also used for the staining in reticulocytes, where the dye stains reticulin.

Adéquation

Certified as a supravital stain for reticulocytes.

Autres remarques

The structure of Brilliant Cresyl Blue ALD contains a 2-methyl modification on the phenoxazine core, while the structure of the historic Brilliant Cresyl Blue contains a 8-methyl modification on the phenoxazine core. The biological staining application is the same for both structures.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Gloves