B9673

1-Bromo-3-chloropropane

for isolation of RNA

• Synonyme(s) : Chlorure bromure de triméthylène, I-BCP
• Formule linéaire : Cl(CH₂)₃Br
• CAS Number: 109-70-6
• Poids moléculaire : 157.44
• Beilstein: 605278
• Numéro CE : 203-697-1
• Numéro MDL: MFCD00000998
• Code UNSPSC : 41105515
• ID de substance PubChem : 24892149
• Nomenclature NACRES : NA.52

Propriétés

• Qualité: Molecular Biology
• Niveau de qualité: 200
• Essai: ≥98.5% (GC)
• Forme: liquid
• Indice de réfraction: n20/D 1.486 (lit.)
• pb: 144-145 °C (lit.)
• Densité: 1.592 g/mL at 25 °C (lit.)
• Température de stockage: room temp
• Chaîne SMILES: ClCCCBr
• InChI: 1S/C3H6BrCl/c4-2-1-3-5/h1-3H2
• Clé InChI: MFESCIUQSIBMSM-UHFFFAOYSA-N

Description

Description générale

BCP can be used in place of chloroform and is less toxic. The use of BCP in the single-step TRI reagent method decreases the possibility of contaminating RNA with DNA. It does not adversely affect quality or quantity of the isolated RNA. 1-Bromo-3-chloropropane is a phase separation reagent used in protocols for isolation of nucleic acids.

Application

1-Bromo-3-chloropropane has been used in molecular biology applications for the isolation of high quality RNA from:

• feline teeth
• mice brain and liver tissues
• human ectocervical epithelial cells
• placental samples
• human mesenchymal stromal cells (hMSCs)

Caractéristiques et avantages

• less toxic alternative to chloroform
• compatible with TRI reagent for isolation of RNA with decreased DNA contamination

Autres remarques

BCP can be used in place of chloroform, and is less toxic. The use of BCP in the single-step TRI Reagent method decreases the possibility of contaminating RNA with DNA. It does not adversely affect quality or quantity of the isolated RNA.

Informations sur la sécurité

• Pictogrammes: GHS06,GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H302,H331,H335,H341,H412
• Conseils de prudence: P202 - P261 - P273 - P301 + P312 - P304 + P340 + P311 - P308 + P313
• Classification des risques: Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Muta. 2 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Classe de danger pour l'eau (WGK): WGK 2
• Équipement de protection individuelle: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter