C0412

Concanavalin A from Canavalia ensiformis (Jack bean)

Type IV-S, lyophilized powder, γ-irradiated, BioReagent, suitable for cell culture

• Synonyme(s) : Con A
• CAS Number: 11028-71-0
• Numéro CE : 234-258-2
• Numéro MDL: MFCD00071069
• Code UNSPSC : 23151817
• Nomenclature NACRES : NA.32

Propriétés

• Niveau de qualité: 200
• Stérilité: γ-irradiated
• Type: Type IV-S
• Gamme de produits: BioReagent
• Forme: lyophilized powder
• Technique(s): cell culture | mammalian: suitable
• Impuretés: salt, essentially free
• Solubilité: PBS: 5 mg/mL, slightly hazy; H₂O: soluble, slightly hazy
• Température de stockage: −20°C

Description

Application

Concanavalin A from Canavalia ensiformis (jack bean) has been used:

• to induce spleen blastogenesis
• to generate ConA blast targets from B6 and lpr spleen cells
• to stimulate splenic lymphocytes from estrogen-and placebo-treated mice

Actions biochimiques/physiologiques

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca²⁺ and Mn²⁺ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

Remarque sur l'analyse

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca²⁺ and Mn²⁺ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H317,H334,H361
• Conseils de prudence: P202 - P261 - P272 - P280 - P302 + P352 - P308 + P313
• Classification des risques: Repr. 2 - Resp. Sens. 1 - Skin Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Faceshields, Gloves