C0794

Chitosanase from Streptomyces sp.

buffered aqueous glycerol solution, ≥15 units/mg protein (E^1%)

• Synonyme(s) : Chitosan N-acetylglucosaminohydrolase
• CAS Number: 51570-20-8
• Numéro CE : 3.2.1.132 (BRENDA, IUBMB)
• Numéro MDL: MFCD00211797
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Forme: buffered aqueous glycerol solution
• Niveau de qualité: 200
• Activité spécifique: ≥15 units/mg protein (E^1%)
• Concentration: ≥0.5 mg/mL
• Solubilité: 0.1 M acetate buffer, pH 5.5: soluble
• Température de stockage: −20°C

Description

Application

Chitosanase from Sigma has been used for polarized light-stimulated, enzymatic hydrolysis of chitin and chitosan.

Actions biochimiques/physiologiques

Chitosanase catalyzes the endohydrolysis of β-1,4-linkages between D-glucosamine (GlcN-GlcN) residues in chitosan. The enzyme from Streptomyces has been reported to also hydrolyze the GlcNAc-GlcN linkage in partially acetylated chitosan.

Définition de l'unité

One unit will liberate 1.0 μmole of reducing sugar (measured as D-glucosamine equivalents) from chitosan per minute at pH 5.5 at 37 °C.

Forme physique

Solution in 50% glycerol containing 50 mM sodium acetate

Notes préparatoires

Soluble in 10mL of 0.1 M Acetate buffer at pH 5.5

Autres remarques

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable