C0888

Clostripain from Clostridium histolyticum

≥20 units/mg solid

• Synonyme(s) : Clostridiopeptidase B, Proteinase from Clostridium histolyticum
• CAS Number: 9028-00-6
• Numéro CE : 3.4.22.8 (BRENDA, IUBMB)
• Numéro CE : 232-822-2
• Numéro MDL: MFCD00130814
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Forme: lyophilized powder
• Niveau de qualité: 100
• Activité spécifique: ≥20 units/mg solid
• Poids mol.: 15.4 kDa; 41.7 kDa
• Impuretés: salt, essentially free
• Température de stockage: 2-8°C

Description

Application

Clostripain from Clostridium histolyticum has been used as a proteolytic enzyme in perfusate to detect its effect on tube hematocrit. It has also been used in limited proteolysis of DNA polymerase (gp43) of phage T4 (RB69 gp43).

Actions biochimiques/physiologiques

Clostripain from Clostridium histolyticum is composed of two polypeptide chains, with molecular masses of 41.7 kDa and 15.4 kDa. Clostripain has a highly restricted substrate specificity for Arg-Xaa peptide bonds. Therefore, clostripain has been explored as a potential enzyme for protein sequencing purposes. It has also been studied as a catalyst for condensation of pharmaceutically important peptides containing Arg-Pro bonds.

Conditionnement

Bottomless glass bottle. Contents are inside inserted fused cone.

Qualité

Purified, essentially salt-free

Définition de l'unité

One unit will hydrolyze 1.0 μmole of BAEE per min at pH 7.6 at 25 °C in the presence of 2.5 mM DTT.

Remarque sur l'analyse

The enzyme must be activated for 2-3 hours before use by dissolving in 2.5 mM dithiothreitol containing 1.0 mM calcium acetate.

Informations sur la sécurité

• Pictogrammes: GHS08,GHS07
• Mention d'avertissement: Danger
• Mentions de danger: H315,H319,H334,H335
• Conseils de prudence: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Classification des risques: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)