C3735

Cytochrome P450 human

1A1 Isozyme Microsomes, with P450 Reductase, recombinant, expressed in baculovirus infected insect cells (BTI-TN-5B1-4)

• Numéro CE : 1.6.2.4 (BRENDA, IUBMB)
• Numéro MDL: MFCD03456520
• Code UNSPSC : 12161501
• Nomenclature NACRES : NA.47

Propriétés

• Source biologique: human
• Niveau de qualité: 200
• Produit recombinant: expressed in baculovirus infected insect cells (BTI-TN-5B1-4)
• Forme: solution
• Activité spécifique: ≥4 units/pmol enzyme
• Poids mol.: 45-60 kDa
• Conditionnement: vial of 0.5 nmol
• Technique(s): activity assay: suitable
• Solubilité: water: soluble
• Adéquation: suitable for molecular biology
• Numéro d'accès UniProt: P04798
• Application(s): cell analysis
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... CYP1A1(1543)

Description

Description générale

Research area: Immuno and CKS

Cytochrome P450 (CYP) enzymes are a family of enzymes that are encoded by the P450 genes. These enzymes are membrane bound hemoproteins. that are mainly found in the liver’s endoplasmic reticulum.

Actions biochimiques/physiologiques

Cytochrome P450 is a heterogeneous family of isozymes whose primary function is to oxidize small molecules, both as a function of intermediary metabolism (e.g., fatty acids) and to detoxify exogenous compounds (drugs or toxins). Some isoforms have narrow substrate specificity, while others are promiscuous. The CYP1A1 isoform catalyzes 7-deethylation of ethoxyresorufin. Cytochrome P450 (CYP) plays an important role in detoxifying xenobiotics, cellular metabolism and homeostasis. One of the main mechanisms of drug-drug interactions is the induction or inhibition of these enzymes. CYP enzymes are transcriptionally activated by a variety of xenobiotics and by endogenous substrates via receptor-dependent pathways. Inhibition of these enzymes is a major factor in metabolism-based drug-drug interactions, and many chemotherapeutic medications can cause drug interactions by either inhibiting or inducing the cytochrome p450 enzyme system.

Forme physique

Solution in 100 mM potassium phosphate buffer, pH 7.4.

Notes préparatoires

Microsomes containing human CYP1A1 and recombinant human NADPH-P450 reductase.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable