C3888

Carboxypeptidase Y from baker′s yeast (S. cerevisiae)

lyophilized powder, ≥50 units/mg protein

• Synonyme(s) : Peptidyl-L-amino acid Hydrolase, Serine Carboxypeptidase
• CAS Number: 9046-67-7
• Numéro CE : 3.4.16.1 (BRENDA, IUBMB)
• Numéro MDL: MFCD00130723
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Qualité: Proteomics Grade
• Niveau de qualité: 200
• Forme: lyophilized powder
• Activité spécifique: ≥50 units/mg protein
• Poids mol.: 61 kDa
• Composition: Protein, ≥75% E1%/280
• Conditions d'expédition: wet ice
• Température de stockage: −20°C

Description

Application

Carboxypeptidase Y has a broad specificity and is stable in urea. Hence, this enzyme is not like other carboxypeptidases and can be used for sequence analysis. Due to its amidase action, this enzyme might be applied to the sequence analysis of peptides having amidated COOH-terminal groups such as oxytocin and vasopressin.

Actions biochimiques/physiologiques

The glycoprotein has a molecular weight of about 61,000, has a nitrogen content of 12.74%. It is a single polypeptide chain of 442 residues with 16 residues of glucosamine in the carbohydrate moiety. Lysine is at the NH2 terminus and -Asp-Ser-Thr-Leu is the COOH-terminal sequence. The principal action of the enzyme is to remove COOH-terminal residues from polypeptide chains. It is used as a vacuolar marker enzyme for studies on protein transport and localization.

Conditionnement

Package size based on protein content

Définition de l'unité

One unit will hydrolyze 1.0 μmole of N-CBZ-Phe-Ala to N-CBZ-L-phenylalanine and L-alanine per min at pH 6.75 at 25°C, based on EM/230 = 191.5.

Forme physique

Lyophilized powder containing citric acid.

Remarque sur l'analyse

amidase and esterase activities may be present

Informations sur la sécurité

• Pictogrammes: GHS08,GHS07
• Mention d'avertissement: Danger
• Mentions de danger: H315,H319,H334,H335
• Conseils de prudence: P302 + P352 - P305 + P351 + P338
• Classification des risques: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable