C4018

Choloylglycine Hydrolase from Clostridium perfringens (C. welchii)

lyophilized powder, ≥100 units/mg protein

• Synonyme(s) : 3α,7α,12α-Trihydroxy-5β-cholan-24-oylglycine amidohydrolase
• CAS Number: 37289-07-9
• Numéro CE : 3.5.1.24 (BRENDA, IUBMB)
• Numéro MDL: MFCD00130789
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Forme: lyophilized powder
• Niveau de qualité: 200
• Activité spécifique: ≥100 units/mg protein
• Composition: Protein, ≥50% biuret
• Conditions d'expédition: wet ice
• Température de stockage: −20°C

Description

Application

The enzyme from Sigma has been used in the analysis of bile samples in various studies.

Actions biochimiques/physiologiques

The enzyme is an N-terminal nucleophilic (Ntn) hydrolase that catalyzes the hydrolysis of amide bonds, liberates the glycine/taurine moiety from the steroid core and eventually yields unconjugated bile acids. Agents that oxidize thiol groups (e.g., p-mercuribenzoate, iodoacetamide, Hg²⁺, Cu²⁺, and Cd²⁺) have been shown to strongly inhibit bile salt hydrolase (BSH) activity in Clostridium perfringens.

Définition de l'unité

One unit will hydrolyze 1.0 μmole of glycocholic acid to glycine and cholic acid in 5 min at pH 5.6 at 37 °C.

Forme physique

Partially purified lyophilized powder containing buffer salts and stabilizer

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)