C4749

Carboxylesterase 2 human

recombinant, expressed in mouse NSO cells, ≥95% (SDS-PAGE)

• Synonyme(s) : CES2, CES2A1
• Numéro CE : 3.1.1.1 (BRENDA, IUBMB)
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Produit recombinant: expressed in mouse NSO cells
• Niveau de qualité: 200
• Essai: ≥95% (SDS-PAGE)
• Forme: solution
• Activité spécifique: ≥1.0 EU/μg; 30,000 pmol/min-μg protein
• Poids mol.: predicted mol wt ~60 kDa
• Concentration: 0.4-0.6 mg/mL
• Impuretés: ≤1.0 EU/μg endotoxin
• Numéro d'accès NCBI: NM_003869; NP_003860
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... CES2(8824)

Description

Actions biochimiques/physiologiques

Human carboxylesterase 2 (hCE-2) recognizes a substrate with a large alcohol group and small acyl group. Its substrate specificity may be restricted by a capability of acyl-hCE-2 conjugate formation due to the presence of conformational interference in the active site pocket. Carboxylesterases catalyze the biotransformation of several ester-containing drugs and prodrugs such as angiotensin-converting enzyme inhibitor (temocarpil, cilazapril), anti-tumor drugs (capecitabin) and narcotics.

Member of a serine esterase family that hydrolyze ester and amide bonds. Carboxylesterase 2 is an endoplasmic reticulum-bound hydrolase that plays a critical role in xenobiotic detoxification and activation for ester-containing therapeutics. Carboxylesterase 2 is also involved in the detoxification of drugs such as heroin and cocaine. This enzyme is thought to play a role in lipid metabolism.

Définition de l'unité

One unit will cause the hydrolysis of 1 picomole of p-nitrophenylacetate per minute at pH 7.5 at 25 deg C.

Forme physique

Supplied as a solution containing sodium chloride, sodium acetate, and 20% glycerol.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable