C8726

Caspase 9 human

≥90% (SDS-PAGE), recombinant, expressed in E. coli (C-terminal histidine-tagged), buffered aqueous solution, >2,000 units/mg protein

• Synonyme(s) : ICE-Lap6, Mch6
• Numéro CE : 3.4.22.62 (BRENDA, IUBMB)
• Numéro MDL: MFCD02687006
• Code UNSPSC : 12352204

Propriétés

• Produit recombinant: expressed in E. coli (C-terminal histidine-tagged)
• Niveau de qualité: 200
• Essai: ≥90% (SDS-PAGE)
• Forme: buffered aqueous solution
• Activité spécifique: >2,000 units/mg protein
• Poids mol.: N-terminal prodomain plus the large subunit. 36 kDa (caspase 9 expressed as a C-terminal histidine-tagged protein appears as a two-subunit protein); small subunit 13 kDa (subunit containing the histidine tag)
• Numéro d'accès UniProt: P55211
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... CASP9(842)

Description

Actions biochimiques/physiologiques

Activation of caspase-9 (CASP9) through apoptotic stimuli initiates the caspase cascade. Caspases have been implicated in many disorders including cancer, inflammatory disease, neurodegenerative diseases, stroke and myocardial infarction.

When cells receive apoptotic stimuli, such as activation of the TNFα/Fas cell surface receptor, caspase 8 activation, Bid processing and its translocation to the mitochondria ensue. As a result mitochondria release cytochrome c which then binds to Apaf-1, the mammalian Ced-4 homologue, together with dATP. The resultant complex recruits caspase 9 leading to its activation. It then cleaves downstream caspases such as caspase 3, 6, and 7, initiating the caspase cascade. Caspase 9 exhibits basal activity. It can be further activated via interaction of its N-terminal prodomain with the activator protein Apaf-1 in the presence of cytochrome c and dATP. Caspases have been implicated in many disorders including cancer, inflammatory disease, neurodegenerative diseases, stroke and myocardial infarction.

Définition de l'unité

One unit will cleave 1.0 nmol of Ac-Leu-Glu-His-Asp-AFC per hr at 25 °C at pH 6.5.

Forme physique

Solution in 10% sucrose containing 50 mM HEPES, pH 7.5, 5 mM DTT, 0.1% CHAPS, 1 mM EDTA, 50 mM NaCl.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)