C9098
Milieu CHO
Animal-component free, without L-glutamine, use at 21.5 g/L, dry powder, suitable for cell culture
Synonyme(s) :
CHO Medium
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About This Item
Produits recommandés
Niveau de qualité
Description
for research or for further manufacturing use
Forme
dry powder
Qualité
Drug/Device Master File available
Concentration
21.5 g/L
Technique(s)
cell culture | mammalian: suitable
Température de stockage
2-8°C
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Caractéristiques et avantages
Developed to meet the needs of biotechnology and vaccine manufacturing, this medium supports rapid initial cell growth and high levels of protein expression in suspension cultures. It also supports high cell densities and maintenance of these densities of viable cells for extended periods resulting in increased productivity.
Autres remarques
Proprietary formulation containing inorganic salts, HEPES, essential and non-essential amino acids, vitamins, recombinant human insulin, plant hydrolysates, other organic compounds, trace elements, and surfactants.
Does not contain antibiotics, antimycotics, L-glutamine, or transferrin. Contains no animal-derived proteins or other components.
Does not contain antibiotics, antimycotics, L-glutamine, or transferrin. Contains no animal-derived proteins or other components.
Animal component-free medium formulated to optimize cell growth and protein expression in Chinese hamster ovary (CHO) cells.
Reconstitution
Powdered media are extremely hygroscopic and should be protected from atmospheric moisture. Preparing a concentrated solution of medium is not recommended as precipitates may form.
1. Measure out 90% of final required volume of tissue culture grade water. Water temperature should be 25-40oC.
2. While stirring the water, add the powdered medium (21.5 g/L). Heat may enhance solubility but do not go above 40oC.
3. Rinse original package with a small amount of water to remove all traces of the powder. Add to solution in step 2.
4. Stir until dissolved.
5. Add 0.6g/L (range 0.6-1.2g/L or higher depending on individual cell line) of G3126 L-Glutamine.
6. Add 1.5 g/L of S8875 sodium bicarbonate (If increased buffering capacity is desired this amount can be increased to 3.0 g/L).
7. Adjust the pH to 7.45 range (7.3-7.6) using 5 N NaOH.
8. Use additional water to bring the solution to final volume.
9. Sterilize immediately by filtration using a membrane with a porosity of <0.22 microns. Do not use poly-ether sulfones (PES) type of membranes. We recommend PVDF as the best inert type of filter membrane.
10. Aseptically dispense medium into a sterile container. Store at 2-8 °C in the dark.
1. Measure out 90% of final required volume of tissue culture grade water. Water temperature should be 25-40oC.
2. While stirring the water, add the powdered medium (21.5 g/L). Heat may enhance solubility but do not go above 40oC.
3. Rinse original package with a small amount of water to remove all traces of the powder. Add to solution in step 2.
4. Stir until dissolved.
5. Add 0.6g/L (range 0.6-1.2g/L or higher depending on individual cell line) of G3126 L-Glutamine.
6. Add 1.5 g/L of S8875 sodium bicarbonate (If increased buffering capacity is desired this amount can be increased to 3.0 g/L).
7. Adjust the pH to 7.45 range (7.3-7.6) using 5 N NaOH.
8. Use additional water to bring the solution to final volume.
9. Sterilize immediately by filtration using a membrane with a porosity of <0.22 microns. Do not use poly-ether sulfones (PES) type of membranes. We recommend PVDF as the best inert type of filter membrane.
10. Aseptically dispense medium into a sterile container. Store at 2-8 °C in the dark.
Informations légales
EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany
Supplément
Réf. du produit
Description
Tarif
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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