CMC0014

BL21(DE3) Chemically Competent Cells

Escherichia coli, rod shaped

• Synonyme(s) : Competent E. coli cells
• Code UNSPSC : 41106202
• Nomenclature NACRES : NA.85

Propriétés

• Product Name: BL21(DE3) Chemically Competent Cells, for protein expression
• Source biologique: Escherichia coli
• Qualité: Molecular Biology
• Mode de croissance: adherent or suspension
• Morphologie: rod shaped
• Technique(s): microbiological culture: suitable
• Transformation cellulaire: competent cell type: chemically competent; transformation efficiency: ≥1 x 10⁷ cfu/μg
• Conditions d'expédition: dry ice
• Température de stockage: −70°C

Description

Description générale

The BL21(DE3) Chemically Competent strains contain the phage T7 RNA polymerase gene linked to the IPTG-inducible promoter, for use with any expression plasmid containing the T7 promoter.

Genotype

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

Application

The E. coli strain BL21 (DE3) has been used:

• to express tau 2N4R isoform (aa1–441) and the truncated tau (aa1–421) using pET19b as expression vector.
• BL21(DE3)Chemically Competent Cells has been used for the transformation and expressionof protegrin-1 (PG1) peptides.
• to express 6His-TEVsite-Hs ARF1 (17-181)-Q71L in pHis2 vector.

Caractéristiques et avantages

• a transformation efficiency of >1 × 10⁷ cfu/μg.
• economical prices
• offering superior value for everyday protein expression work.

Composants

• BL21(DE3) chemically competent cells
• pUC 19 transformation control DNA
• recovery medium for expression

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids