D0428

1 kb DNA Ladder

for DNA electrophoresis

• Synonyme(s) : 1kb gel ladder, 1kb gel marker, 1kb ladder for gel electrophoresis, DNA gel marker, agarose gel electrophoresis ladder, agarose gel electrophoresis marker
• Code UNSPSC : 41105335
• Nomenclature NACRES : NA.25

Propriétés

• Niveau de qualité: 100; 200
• Forme: liquid
• Adéquation: suitable for electrophoresis (DNA)
• Température de stockage: −20°C

Description

Description générale

Sigma′s 1 kb Ladder contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb, 1 kb repeats from 3 to 6 kb, and 2 kb repeats from 6 to 10 kb. In nucleic acid gel electrophoresis, five μl of the marker should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel. Suitable for use in Northern and Southern blotting. All DNA markers can be stained with ethidium bromide, SYBR^® Green, and Nancy-520.

Application

1 kb DNA Ladder has been used as a molecular marker to determine the molecular weight and size of double stranded DNA during gel electrophoresis.

Composants

Sigma′s 1kb Ladder is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Autres remarques

For optimal resolution, the recommended agarose gel concentration is 0.75%.

Informations légales

SYBR is a registered trademark of Life Technologies

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)