D0690

DNA Gyrase from Escherichia coli

aqueous glycerol solution

• Numéro CE : 5.99.1.3 (BRENDA, IUBMB)
• Numéro MDL: MFCD00211877
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: Escherichia coli
• Niveau de qualité: 200
• Forme: aqueous glycerol solution
• Poids mol.: ~374 kDa
• Concentration: ≥2 unit/μL
• Technique(s): cell based assay: suitable
• Numéro d'accès UniProt: P0AES4; P0AES6
• Application(s): cell analysis
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: Escherichia coli K12 ... gyrA(946614), gyrB(948211)

Description

Application

DNA gyrase from Escherichia coli has been used in a study to investigate a comparative proteomic approach to better define Deinococcus nucleoid specificities. DNA gyrase from Escherichia coli has also been used in a study to investigate the role of the DnaK-ClpB bichaperone system in DNA gyrase reactivation.

Actions biochimiques/physiologiques

Can be used to supercoil plasmids.

DNA gyrase is supplied as an A₂B₂ holoenzyme. The molecular mass of subunit A is 97 kDa and that of subunit B is 90 kDa. It catalyzes the ATP-dependent introduction of negative supercoils into relaxed DNA. DNA gyrase has been successfully converted into a type II topoisomerase by mutagenesis experiments.

Définition de l'unité

One unit of gyrase activity will supercoil 0.5 micrograms of pBR-322 DNA in 30 minutes at 37 °C.

Autres remarques

Solution in 50% Glycerol containing Tris buffer, DTT and EDTA.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable