D218

Monoclonal Anti-Dihydropyridine Receptor (α₁ Subunit) antibody produced in mouse

clone 1A, buffered aqueous solution

• Numéro MDL: MFCD01633297
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Conjugué: unconjugated
• Niveau de qualité: 200
• Forme d'anticorps: ascites fluid
• Type de produit anticorps: primary antibodies
• Clone: 1A, monoclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~200 kDa
• Espèces réactives: human (weakly), guinea pig, mouse, rat, rabbit
• Technique(s): immunohistochemistry (frozen sections): 1:200; immunoprecipitation (IP): suitable; western blot (chemiluminescent): 1:500
• Isotype: IgG1
• Numéro d'accès UniProt: Q13698
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... CACNA1S(779) ; mouse ... Cacna1s(12292) ; rat ... Cacna1s(682930)

Description

Description générale

DHP receptor is composed of at least four subunits. The α1 subunit contains the binding site for the DHPs and shows high sequence homology to the voltage gated Na+ channel. The α2 subunit is a large glycoprotein and the other 2 subunits are termed β and γ.

Spécificité

Reacts specifically with the α₁ subunit of the DHP receptor. May be used to inhibit the DHP-sensitive calcium current in BC3H1 mouse muscle cells. By immunohistochemistry, double rows of discrete punctate staining represent pairs of triads on the opposing sides of the Z-lines.

Immunogène

rabbit muscle transverse tubule dihydropyridine (DHP) receptors.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Monoclonal Anti-Dihydropyridine Receptor (α1 Subunit) antibody produced in mouse is suitable for immunoprecipitation, immunohistochemistry at a working dilution of 1:500 and immunoblotting at 1:2000. It was used for the detection and localization of DHPR-1α by western blotting in skeletal muscle tissue sections of mice in a study. The antibody may be used to inhibit the DHP-sensitive calcium current in BC3H1 mouse muscle cells.

Actions biochimiques/physiologiques

The 1,4-dihydropyridines (DHPs) are synthetic organic compounds which can be used to identify the L-type Ca²⁺ channels that are found in all types of vertebrate muscle, neuronal and neuroendocrine cells. DHP receptor is part of the L-type calcium channel complex and is thought to be the voltage sensor in E-C coupling.

Forme physique

Solution in phosphate buffered saline containing 0.05% sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)