D6883

2′,7′-Dichlorodihydrofluorescein diacetate

≥97% (HPLC), powder, cell-permeable non-fluorescent probe

• Synonyme(s) : 2′,7′-Dichlorofluorescin diacetate, DCFH-DA, 2′,7′-Dichlorodihydrofluorescein diacetate
• Formule empirique (notation de Hill) : C₂₄H₁₆Cl₂O₇
• CAS Number: 4091-99-0
• Poids moléculaire : 487.29
• Beilstein: 4610273
• Numéro MDL: MFCD00128955
• Code UNSPSC : 12352200
• ID de substance PubChem : 24894058
• Nomenclature NACRES : NA.77

Propriétés

• Product Name: 2′,7′-Dichlorodihydrofluorescein diacetate, ≥97%
• Niveau de qualité: 200
• Essai: ≥97%
• Forme: powder
• Température de stockage: −20°C
• Chaîne SMILES: CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C(c2cc1Cl)c4ccccc4C(O)=O
• InChI: 1S/C24H16Cl2O7/c1-11(27)31-21-9-19-15(7-17(21)25)23(13-5-3-4-6-14(13)24(29)30)16-8-18(26)22(32-12(2)28)10-20(16)33-19/h3-10,23H,1-2H3,(H,29,30)
• Clé InChI: PXEZTIWVRVSYOK-UHFFFAOYSA-N

Description

Description générale

2′,7′-Dichlorodihydrofluorescein diacetate (DCFH-DA) is an oxidation-sensitive fluorescent dye used to detect the intracellular reactive oxygen species (ROS) level. DCFH-DA is a cell-permeable non-fluorescent probe, that can be hydrolyzed by intracellular esterase to DCFH, which forms fluorescent DCF when oxidized by the intracellular ROS.

The excitation wavelength is 504 nm and emission wavelength is 529 nm in ethanol. The use of DMSO as the solvent has not been determined, however various publications suggest that it is suitable.

Application

2′,7′-Dichlorofluorescin diacetate is suitable for use in:

• sensitive and rapid quantitation of oxygen-reactive species in response to oxidative metabolism
• microplate assay for detecting oxidative products in phagocytic cells
• quantitative multiwell myeloid differentiation assay

Applications include sensitive and rapid quantitation of oxygen-reactive species in response to oxidative metabolism; microplate assay for detecting oxidative products in phagocytic cells, and quantitative multiwell myeloid differentiation assay.

Stockage et stabilité

This product should be stored in powdered format at -20°C.

Autres remarques

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Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)