D8764

Deoxyribonuclease II from bovine spleen

Type V, essentially salt-free, lyophilized powder, ≥1,000 units/mg protein

• Synonyme(s) : DNase II, Deoxyribonucleate 3′-oligonucleotido-hydrolase
• CAS Number: 9025-64-3
• Numéro CE : 3.1.22.1 (BRENDA, IUBMB)
• Numéro CE : 232-801-8
• Numéro MDL: MFCD00130919
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: bovine spleen
• Niveau de qualité: 200
• Type: Type V
• Forme: essentially salt-free, lyophilized powder
• Activité spécifique: ≥1,000 units/mg protein
• Poids mol.: 38 kDa
• Concentration: ≥50% protein
• Technique(s): DNA extraction: suitable; tissue culture: suitable
• Adéquation: suitable for molecular biology
• Application(s): cell analysis
• Activité étrangère: RNase ≤0.1%
• Température de stockage: −20°C

Description

Application

Deoxyribonuclease II from bovine spleen has been used:

• as a component of digestion mix to isolate immune cells from colon samples
• to digest retinal explants for single-cell suspension preparation
• for isolation of stromal cells from human colon tissue
• in the dissociation medium during the preparation of embryonic cardiac myocytes from rat heart

Actions biochimiques/physiologiques

Deoxyribonuclease II, also called as acid DNAse, hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3′-phosphates. in vitro, its optimum pH range is 4.5 - 5.0. It also acts upon p-nitrophenyl-phosphodiesters at pH 5.6 - 5.9. The molecular weight is approximately 38,000 Da.

Définition de l'unité

One Kunitz unit will produce a ΔA₂₆₀ of 0.001 per min per mL at pH 4.6 at 25 °C; [Mg²⁺] = 0.83 mM

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable