E5529

EZview^™ Red Streptavidin Affinity Gel

• Code UNSPSC : 41106500
• Nomenclature NACRES : NA.56

Propriétés

• Forme: suspension
• Niveau de qualité: 200
• Durée de conservation: 1 yr
• Technique(s): western blot: suitable
• Matrice: 4% agarose
• pH: 7.2
• Capacité: ~10 μg(biotin per ml of packed gel)
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C

Description

Description générale

The EZ View Red Streptavidin Affinity gel is composed of streptavidin, covalently attached to
cyanogen bromide-activated 4% agarose beads. It is designed to capture (pull-down) the biotinylated target molecules, such as proteins, peptides, antibodies, nucleic acids, lectins, receptors and ligands.

Application

Sutitable for use with immunoprecipitation, western blotting,and enzyme assays.

When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview^™ Red Affinity Gels greatly reduce the risk of pellet loss. EZview^™ resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.

Caractéristiques et avantages

• Increased visibility - Red color reduces risk of incidental aspiration
• Improved recovery of target protein by reduced accidental loss
• Higher reproducibility - More consistent yields

Forme physique

1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon

Informations légales

EZview is a trademark of Sigma-Aldrich Co. LLC

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable