F4021

Follicle Stimulating Hormone from human pituitary

~7,000 IU/mg (powder)

• Synonyme(s) : FSH, Follitropin
• CAS Number: 9002-68-0
• Numéro MDL: MFCD00131099
• Code UNSPSC : 51111800
• eCl@ss : 42020504
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human pituitary glands
• Niveau de qualité: 200
• Stérilité: non-sterile
• Forme: powder
• Activité spécifique: ~7,000 IU/mg (powder)
• Poids mol.: 35 kDa
• Technique(s): cell culture | mammalian: suitable
• Impuretés: HBsAg, HCV and HIV-1/HIV-2, tested negative
• pH: 7.4
• Solubilité: water: ~50 g/L
• Numéro d'accès UniProt: P01225; P23945
• Conditions d'expédition: ambient
• Température de stockage: −20°C
• Informations sur le gène: human ... FSHB(2488), FSHR(2492)

Description

Description générale

The α chain of FSH has 89 amino acids and the β chain has 115 amino acids. The α chain is not active. FSH belongs to glycoprotein hormone family. It is secreted by the gonadotropic cells of pituitary gland.
Research area: Cell signaling

Application

Follicle stimulating hormone (FSH) from human pituitary has been used for-

• in vitro luteinization of Macaque granulosa cells
• luteal cell proliferation assay
• culturing of preantral follicle cells obtained from mice
• in vitro maturation of immature cumulus-oocyte complexes (COCs) obtained from mice and
• culture of RAW264.7 cell line

Actions biochimiques/physiologiques

FSH (follicle stimulating hormone) in involved in the growth of ovarian follicles and steroidogenesis of ovaries. FSHR (follicle-stimulating hormone receptor) interacts with FSH in the ovaries and determines the responsiveness to this hormone. Depressed levels of FSHR result in poor responsiveness and influence folliculogenesis. In the Caucasian population, certain polymorphisms in the FSHR gene are more commonly found in patients with polycystic ovary syndrome (PCOS). In the male fetuses and neonates, it facilitates the proliferation of Sertoli cells. In pubescent males, it stimulates spermatogonia to undergo mitosis.FSH upon binding to its receptor FSH-R positively regulates G-protein coupled receptors/cyclic adenosine monophosphate (cAMP)/protein kinase A pathways to cascade intracellular signals.

The α-chain is not active; biological specificity is attributed to the β-chain. Induces maturation of Graafian follicles of the ovary; promotes development of germinal cells in males; activates cytosolic tyrosine kinase.

Conditionnement

Package size based on protein content

Autres remarques

Two-chain glycoprotein hormone

Qualité

Vial also contains 0.1 mg of salts from 0.05 M phosphate buffer, pH 7.4.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable